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Assay Detail

CHEMBL4042616

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at TEV protease cleavage site linked GAL4-VP16-fused GPR35 in human Tango GPR35-bla U2OS cells assessed as induction of TEV protease-tagged beta-arrestin recruitment incubated for 5 hrs by beta-lactamase reporter gene assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled receptor 35 (CHEMBL1293267)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2H-Chromen-2-one Derivatives as G Protein-Coupled Receptor-35 Agonists.
Wei L, Wang J, Zhang X, Wang P, Zhao Y, Li J, Hou T, Qu L, Shi L, Liang X, Fang Y.
J Med Chem (2017) 60 : 362 -372
PubMed 27976894 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 5.78 6.71

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4100677 1 6.71
CHEMBL4079816 1 6.54
CHEMBL4082281 1 6.32
CHEMBL4092954 1 5.81
CHEMBL28079 ZAPRINAST 2.0 1 5.45
CHEMBL4081238 1 5.44
CHEMBL4065291 1 5.15
CHEMBL4103143 1 4.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4100677 EC50 = 197.0 nM 6.71
CHEMBL4079816 EC50 = 288.0 nM 6.54
CHEMBL4082281 EC50 = 479.0 nM 6.32
CHEMBL4092954 EC50 = 1540.0 nM 5.81
CHEMBL28079 ZAPRINAST EC50 = 3520.0 nM 5.45
CHEMBL4081238 EC50 = 3630.0 nM 5.44
CHEMBL4065291 EC50 = 7050.0 nM 5.15
CHEMBL4103143 EC50 = 14060.0 nM 4.85