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Assay Detail

CHEMBL4043572

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Wistar rat heart mitochondrial CPT-1 assessed as reduction in conversion of palmitoyl CoA and L-carnitine into palmitoylcarnitine preincubated for 3 mins followed by L-carnitine and palmitoyl CoA addition measured for 3.5 mins by spectrophotometric analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Carnitine O-palmitoyltransferase 1, muscle isoform (CHEMBL3216)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Development of Fluorinated Analogues of Perhexiline with Improved Pharmacokinetic Properties and Retained Efficacy.
Tseng CC, Noordali H, Sani M, Madhani M, Grant DM, Frenneaux MP, Zanda M, Greig IR.
J Med Chem (2017) 60 : 2780 -2789
PubMed 28277663 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 4.79 5.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1234355 MALONYL-COENZYME A 1 5.92
CHEMBL75880 PERHEXILINE 4.0 1 4.96
CHEMBL4097976 1 4.96
CHEMBL1592481 1 4.85
CHEMBL4077250 1 4.72
CHEMBL1334033 PERHEXILINE MALEATE 4.0 1 4.50
CHEMBL4081530 1 4.35
CHEMBL4068222 1 4.06
CHEMBL4059578 1 -
CHEMBL4079982 1 -
CHEMBL4097557 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1234355 MALONYL-COENZYME A IC50 = 1200.0 nM 5.92
CHEMBL75880 PERHEXILINE IC50 = 11000.0 nM 4.96
CHEMBL4097976 IC50 = 11000.0 nM 4.96
CHEMBL1592481 IC50 = 14000.0 nM 4.85
CHEMBL4077250 IC50 = 19000.0 nM 4.72
CHEMBL1334033 PERHEXILINE MALEATE IC50 = 32000.0 nM 4.50
CHEMBL4081530 IC50 = 45000.0 nM 4.35
CHEMBL4068222 IC50 = 88000.0 nM 4.06
CHEMBL4059578 IC50 = 215000.0 nM -
CHEMBL4079982 IC50 = 475000.0 nM -
CHEMBL4097557 IC50 = 715000.0 nM -