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Assay Detail

CHEMBL4043777

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant His-tagged FGFR1 cytoplasmic domain (308 to 731 residues) expressed in baculovirus using Tyr-4 peptide after 1 hr by FRET-based Z'-Lyte assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fibroblast growth factor receptor 1 (CHEMBL3650)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

2-Oxo-3, 4-dihydropyrimido[4, 5-d]pyrimidinyl derivatives as new irreversible pan fibroblast growth factor receptor (FGFR) inhibitors.
Li X, Guise CP, Taghipouran R, Yosaatmadja Y, Ashoorzadeh A, Paik WK, Squire CJ, Jiang S, Luo J, Xu Y, Tu ZC, Lu X, Ren X, Patterson AV, Smaill JB, Ding K.
Eur J Med Chem (2017) 135 : 531 -543
PubMed 28521156 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 8.70 9.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4100258 1 9.55
CHEMBL4092354 1 9.14
CHEMBL4077029 1 9.04
CHEMBL4092508 1 9.00
CHEMBL4096041 1 8.97
CHEMBL4069509 1 8.97
CHEMBL4088313 1 8.97
CHEMBL4103795 1 8.94
CHEMBL4068733 1 8.93
CHEMBL4079538 1 8.92
CHEMBL4096738 1 8.91
CHEMBL4060079 1 8.88
CHEMBL4102560 1 8.75
CHEMBL4066038 1 8.74
CHEMBL4072483 1 8.71
CHEMBL4099403 1 8.64
CHEMBL4061908 1 8.63
CHEMBL4100110 1 8.51
CHEMBL4064676 1 8.45
CHEMBL2216830 1 8.44
CHEMBL4087420 1 8.34
CHEMBL4073261 1 7.97
CHEMBL4084614 1 6.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4100258 IC50 = 0.28 nM 9.55
CHEMBL4092354 IC50 = 0.73 nM 9.14
CHEMBL4077029 IC50 = 0.91 nM 9.04
CHEMBL4092508 IC50 = 0.99 nM 9.00
CHEMBL4096041 IC50 = 1.06 nM 8.97
CHEMBL4069509 IC50 = 1.07 nM 8.97
CHEMBL4088313 IC50 = 1.06 nM 8.97
CHEMBL4103795 IC50 = 1.16 nM 8.94
CHEMBL4068733 IC50 = 1.18 nM 8.93
CHEMBL4079538 IC50 = 1.21 nM 8.92
CHEMBL4096738 IC50 = 1.23 nM 8.91
CHEMBL4060079 IC50 = 1.32 nM 8.88
CHEMBL4102560 IC50 = 1.77 nM 8.75
CHEMBL4066038 IC50 = 1.81 nM 8.74
CHEMBL4072483 IC50 = 1.97 nM 8.71
CHEMBL4099403 IC50 = 2.28 nM 8.64
CHEMBL4061908 IC50 = 2.33 nM 8.63
CHEMBL4100110 IC50 = 3.08 nM 8.51
CHEMBL4064676 IC50 = 3.54 nM 8.45
CHEMBL2216830 IC50 = 3.65 nM 8.44
CHEMBL4087420 IC50 = 4.59 nM 8.34
CHEMBL4073261 IC50 = 10.7 nM 7.97
CHEMBL4084614 IC50 = 200.0 nM 6.70