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Assay Detail

CHEMBL4045567

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human HCT116 cells assessed as reduction in cell viability incubated for 24 hrs by CCK8 assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HCT-116
Confidence 1 — Organism
Curated By Autocuration

Target

HCT-116 (CHEMBL394)
Type CELL-LINE
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of novel antitumor spirotetrahydrothiopyran-oxindole derivatives as potent p53-MDM2 inhibitors.
Ji C, Wang S, Chen S, He S, Jiang Y, Miao Z, Li J, Sheng C.
Bioorg Med Chem (2017) 25 : 5268 -5277
PubMed 28797774 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.05 5.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4103052 1 5.62
CHEMBL4085100 1 5.37
CHEMBL4087333 1 5.29
CHEMBL4100837 1 5.25
CHEMBL4089755 1 5.10
CHEMBL4067591 1 5.08
CHEMBL4076023 1 5.07
CHEMBL4105189 1 5.07
CHEMBL4068639 1 5.07
CHEMBL4082861 1 5.06
CHEMBL4098502 1 5.03
CHEMBL4080485 1 5.02
CHEMBL4095014 1 5.01
CHEMBL4079450 1 4.98
CHEMBL4097488 1 4.98
CHEMBL4071267 1 4.98
CHEMBL4095297 1 4.76
CHEMBL4074857 1 4.72
CHEMBL2152332 1 4.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4103052 IC50 = 2400.0 nM 5.62
CHEMBL4085100 IC50 = 4300.0 nM 5.37
CHEMBL4087333 IC50 = 5100.0 nM 5.29
CHEMBL4100837 IC50 = 5600.0 nM 5.25
CHEMBL4089755 IC50 = 8000.0 nM 5.10
CHEMBL4067591 IC50 = 8300.0 nM 5.08
CHEMBL4076023 IC50 = 8600.0 nM 5.07
CHEMBL4105189 IC50 = 8500.0 nM 5.07
CHEMBL4068639 IC50 = 8600.0 nM 5.07
CHEMBL4082861 IC50 = 8800.0 nM 5.06
CHEMBL4098502 IC50 = 9300.0 nM 5.03
CHEMBL4080485 IC50 = 9500.0 nM 5.02
CHEMBL4095014 IC50 = 9800.0 nM 5.01
CHEMBL4079450 IC50 = 10400.0 nM 4.98
CHEMBL4097488 IC50 = 10500.0 nM 4.98
CHEMBL4071267 IC50 = 10500.0 nM 4.98
CHEMBL4095297 IC50 = 17300.0 nM 4.76
CHEMBL4074857 IC50 = 19100.0 nM 4.72
CHEMBL2152332 IC50 = 28400.0 nM 4.55