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Assay Detail

CHEMBL4047196

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABCG2 in human H460/MX20 cells assessed as potentiation of mitoxantrone induced antiproliferative activity by measuring mitoxantrone IC50 at 1 uM preincubated for 2 hrs followed by mitoxantrone addition measured after 72 hrs by MTT assay (Rvb = 6.161 +/- 0.172 microM)
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Design, synthesis and biological evaluation of benzamide and phenyltetrazole derivatives with amide and urea linkers as BCRP inhibitors.
Gujarati NA, Zeng L, Gupta P, Chen ZS, Korlipara VL.
Bioorg Med Chem Lett (2017) 27 : 4698 -4704
PubMed 28916341 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 32 6.00 6.45

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4096318 1 6.45
CHEMBL4076705 1 6.36
CHEMBL4095392 1 6.26
CHEMBL3426387 1 6.23
CHEMBL3426388 1 6.23
CHEMBL4091036 1 6.22
CHEMBL410316 FUMITREMORGIN 1 6.21
CHEMBL4078022 1 6.21
CHEMBL4098858 1 6.20
CHEMBL4096070 1 6.19
CHEMBL4064511 1 6.15
CHEMBL4061497 1 6.14
CHEMBL3885456 1 6.13
CHEMBL4074879 1 6.11
CHEMBL4092046 1 6.10
CHEMBL4065537 1 6.08
CHEMBL4083323 1 6.08
CHEMBL4080817 1 6.08
CHEMBL4102382 1 6.04
CHEMBL4099768 1 6.01
CHEMBL4072882 1 5.93
CHEMBL4087699 1 5.91
CHEMBL4077603 1 5.87
CHEMBL4076726 1 5.82
CHEMBL4066351 1 5.79
CHEMBL4104064 1 5.74
CHEMBL4069054 1 5.73
CHEMBL4081645 1 5.60
CHEMBL4102415 1 5.58
CHEMBL4077458 1 5.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4096318 IC50 = 354.0 nM 6.45
CHEMBL4076705 IC50 = 442.0 nM 6.36
CHEMBL4095392 IC50 = 549.0 nM 6.26
CHEMBL3426387 IC50 = 585.0 nM 6.23
CHEMBL3426388 IC50 = 587.0 nM 6.23
CHEMBL4091036 IC50 = 605.0 nM 6.22
CHEMBL410316 FUMITREMORGIN IC50 = 612.0 nM 6.21
CHEMBL4078022 IC50 = 622.0 nM 6.21
CHEMBL4098858 IC50 = 627.0 nM 6.20
CHEMBL4096070 IC50 = 651.0 nM 6.19
CHEMBL4064511 IC50 = 711.0 nM 6.15
CHEMBL4061497 IC50 = 718.0 nM 6.14
CHEMBL3885456 IC50 = 746.0 nM 6.13
CHEMBL4074879 IC50 = 785.0 nM 6.11
CHEMBL4092046 IC50 = 798.0 nM 6.10
CHEMBL4083323 IC50 = 832.0 nM 6.08
CHEMBL4080817 IC50 = 838.0 nM 6.08
CHEMBL4065537 IC50 = 833.0 nM 6.08
CHEMBL4102382 IC50 = 913.0 nM 6.04
CHEMBL4099768 IC50 = 967.0 nM 6.01
CHEMBL4072882 IC50 = 1172.0 nM 5.93
CHEMBL4087699 IC50 = 1221.0 nM 5.91
CHEMBL4077603 IC50 = 1363.0 nM 5.87
CHEMBL4076726 IC50 = 1515.0 nM 5.82
CHEMBL4066351 IC50 = 1615.0 nM 5.79
CHEMBL4104064 IC50 = 1801.0 nM 5.74
CHEMBL4069054 IC50 = 1880.0 nM 5.73
CHEMBL4081645 IC50 = 2526.0 nM 5.60
CHEMBL4102415 IC50 = 2655.0 nM 5.58
CHEMBL4077458 IC50 = 2814.0 nM 5.55
CHEMBL4092211 IC50 = 3371.0 nM 5.47
CHEMBL4084461 IC50 = 3662.0 nM 5.44