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Assay Detail

CHEMBL4049525

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Arabidopsis thaliana HPPD expressed in Escherichia coli T7 expression system using HPPA as substrate preincubated for 30 mins followed by substrate addition measured for 20 mins by HGA dioxygenase enzyme coupled spectrophotometric method
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Arabidopsis thaliana
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

4-hydroxyphenylpyruvate dioxygenase (CHEMBL2242740)
Type SINGLE PROTEIN
Organism Arabidopsis thaliana

Publication

4-Hydroxyphenylpyruvate Dioxygenase and Its Inhibition in Plants and Animals: Small Molecules as Herbicides and Agents for the Treatment of Human Inherited Diseases.
Santucci A, Bernardini G, Braconi D, Petricci E, Manetti F.
J Med Chem (2017) 60 : 4101 -4125
PubMed 28128559 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.92 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1230467 1 7.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1230467 IC50 = 12.0 nM 7.92