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Assay Detail

CHEMBL4050581

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at recombinant human TRPV4 expressed in CHOK1 cells assessed as inhibition of 4alphaPDD-induced activation pretreated for 5 mins followed by 4alphaPDD stimulation measured for 5 mins by FLIPR assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Pharmacological evaluation of novel (6-aminopyridin-3-yl)(4-(pyridin-2-yl)piperazin-1-yl) methanone derivatives as TRPV4 antagonists for the treatment of pain.
Tsuno N, Yukimasa A, Yoshida O, Suzuki S, Nakai H, Ogawa T, Fujiu M, Takaya K, Nozu A, Yamaguchi H, Matsuda H, Funaki S, Yamanada N, Tanimura M, Nagamatsu D, Asaki T, Horita N, Yamamoto M, Hinata M, Soga M, Imai M, Morioka Y, Kanemasa T, Sakaguchi G, Iso Y.
Bioorg Med Chem (2017) 25 : 2177 -2190
PubMed 28284871 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 8.78 9.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4096902 1 9.74
CHEMBL4101768 1 9.72
CHEMBL4066531 1 9.68
CHEMBL3941914 1 9.66
CHEMBL4099293 1 9.54
CHEMBL3971502 1 9.52
CHEMBL4077638 1 9.51
CHEMBL4078117 1 9.41
CHEMBL4095552 1 9.30
CHEMBL4060956 1 9.21
CHEMBL4062935 1 9.19
CHEMBL4084992 1 9.06
CHEMBL4083772 1 9.01
CHEMBL4091558 1 9.00
CHEMBL4080527 1 8.89
CHEMBL4077429 1 8.74
CHEMBL4063752 1 8.70
CHEMBL4083195 1 8.66
CHEMBL4069724 1 8.66
CHEMBL4082835 1 8.62
CHEMBL4090714 1 8.59
CHEMBL4075898 1 8.43
CHEMBL4077357 1 8.41
CHEMBL4069627 1 8.38
CHEMBL4077790 1 8.21
CHEMBL4103303 1 8.20
CHEMBL4090643 1 8.17
CHEMBL2133556 1 7.24
CHEMBL4072343 1 7.19
CHEMBL4076956 1 6.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4096902 IC50 = 0.18 nM 9.74
CHEMBL4101768 IC50 = 0.19 nM 9.72
CHEMBL4066531 IC50 = 0.21 nM 9.68
CHEMBL3941914 IC50 = 0.22 nM 9.66
CHEMBL4099293 IC50 = 0.29 nM 9.54
CHEMBL3971502 IC50 = 0.3 nM 9.52
CHEMBL4077638 IC50 = 0.31 nM 9.51
CHEMBL4078117 IC50 = 0.39 nM 9.41
CHEMBL4095552 IC50 = 0.5 nM 9.30
CHEMBL4060956 IC50 = 0.62 nM 9.21
CHEMBL4062935 IC50 = 0.65 nM 9.19
CHEMBL4084992 IC50 = 0.87 nM 9.06
CHEMBL4083772 IC50 = 0.97 nM 9.01
CHEMBL4091558 IC50 = 0.99 nM 9.00
CHEMBL4080527 IC50 = 1.3 nM 8.89
CHEMBL4077429 IC50 = 1.8 nM 8.74
CHEMBL4063752 IC50 = 2.0 nM 8.70
CHEMBL4083195 IC50 = 2.2 nM 8.66
CHEMBL4069724 IC50 = 2.2 nM 8.66
CHEMBL4082835 IC50 = 2.4 nM 8.62
CHEMBL4090714 IC50 = 2.6 nM 8.59
CHEMBL4075898 IC50 = 3.7 nM 8.43
CHEMBL4077357 IC50 = 3.9 nM 8.41
CHEMBL4069627 IC50 = 4.2 nM 8.38
CHEMBL4077790 IC50 = 6.2 nM 8.21
CHEMBL4103303 IC50 = 6.3 nM 8.20
CHEMBL4090643 IC50 = 6.8 nM 8.17
CHEMBL2133556 IC50 = 57.0 nM 7.24
CHEMBL4072343 IC50 = 64.0 nM 7.19
CHEMBL4076956 IC50 = 170.0 nM 6.77
CHEMBL441472 IC50 - -