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Assay Detail

CHEMBL4051037

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Agonist activity at recombinant human alpha1A adrenergic receptor expressed in HEK293 cells assessed as intercellular Ca mobilization by Fura-2AM dye-based fluorescence assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alpha-1A adrenergic receptor (CHEMBL229)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Novel Indazole Derivatives as Orally Available β3-Adrenergic Receptor Agonists Lacking Off-Target-Based Cardiovascular Side Effects.
Wada Y, Nakano S, Morimoto A, Kasahara KI, Hayashi T, Takada Y, Suzuki H, Niwa-Sakai M, Ohashi S, Mori M, Hirokawa T, Shuto S.
J Med Chem (2017) 60 : 3252 -3265
PubMed 28355078 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 6.54 8.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1437 NOREPINEPHRINE 4.0 1 8.04
CHEMBL4104183 1 6.79
CHEMBL3601200 1 6.66
CHEMBL3601204 1 6.07
CHEMBL4080868 1 5.90
CHEMBL4105097 1 5.75
CHEMBL434 ISOPROTERENOL 4.0 1 -
CHEMBL4089669 1 -
CHEMBL4088750 1 -
CHEMBL4067502 1 -
CHEMBL3601305 1 -
CHEMBL3601307 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1437 NOREPINEPHRINE EC50 = 9.1 nM 8.04
CHEMBL4104183 EC50 = 163.0 nM 6.79
CHEMBL3601200 EC50 = 219.0 nM 6.66
CHEMBL3601204 EC50 = 857.0 nM 6.07
CHEMBL4080868 EC50 = 1250.0 nM 5.90
CHEMBL4105097 EC50 = 1757.0 nM 5.75
CHEMBL434 ISOPROTERENOL EC50 - -
CHEMBL4089669 EC50 > 10000.0 nM -
CHEMBL4088750 EC50 > 10000.0 nM -
CHEMBL4067502 EC50 > 10000.0 nM -
CHEMBL3601305 EC50 > 10000.0 nM -
CHEMBL3601307 EC50 > 10000.0 nM -