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Assay Detail

CHEMBL4051532

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human BRPF2 (563 to 688 residues) expressed in Escherichia coli BL21 (DE3)-R3-pRARE2 preincubated for 15 mins followed by addition of C-terminal biotinylated synthetic K5,12 diacetylated histone H4 (1 to 20 residues) peptide as substrate measured after 1 hr by TR-FRET assay
29
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 1 (CHEMBL2176774)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Benzoisoquinolinediones as Potent and Selective Inhibitors of BRPF2 and TAF1/TAF1L Bromodomains.
Bouché L, Christ CD, Siegel S, Fernández-Montalván AE, Holton SJ, Fedorov O, Ter Laak A, Sugawara T, Stöckigt D, Tallant C, Bennett J, Monteiro O, Díaz-Sáez L, Siejka P, Meier J, Pütter V, Weiske J, Müller S, Huber KVM, Hartung IV, Haendler B.
J Med Chem (2017) 60 : 4002 -4022
PubMed 28402630 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 6.99 7.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4075478 1 7.43
CHEMBL4086958 1 7.29
CHEMBL4104538 1 7.28
CHEMBL4086276 3 7.21
CHEMBL4067968 1 7.21
CHEMBL4076603 1 7.20
CHEMBL4065133 1 7.18
CHEMBL4059651 1 7.16
CHEMBL4064164 1 7.13
CHEMBL4076067 1 7.10
CHEMBL4068180 1 7.09
CHEMBL4091912 1 7.08
CHEMBL4098824 1 7.03
CHEMBL4072812 1 7.03
CHEMBL4092917 1 7.02
CHEMBL4064292 1 7.00
CHEMBL4077317 1 6.99
CHEMBL4080780 1 6.94
CHEMBL4062266 1 6.92
CHEMBL4081145 1 6.91
CHEMBL4063112 1 6.90
CHEMBL4075962 1 6.82
CHEMBL4099171 1 6.61
CHEMBL4064184 1 6.44
CHEMBL4077619 1 6.35
CHEMBL3356143 1 5.96
CHEMBL4067538 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4075478 IC50 = 37.0 nM 7.43
CHEMBL4086958 IC50 = 51.0 nM 7.29
CHEMBL4104538 IC50 = 53.0 nM 7.28
CHEMBL4086276 IC50 = 62.0 nM 7.21
CHEMBL4067968 IC50 = 61.0 nM 7.21
CHEMBL4076603 IC50 = 63.0 nM 7.20
CHEMBL4065133 IC50 = 66.0 nM 7.18
CHEMBL4086276 IC50 = 67.0 nM 7.17
CHEMBL4059651 IC50 = 70.0 nM 7.16
CHEMBL4064164 IC50 = 74.0 nM 7.13
CHEMBL4086276 IC50 = 74.0 nM 7.13
CHEMBL4076067 IC50 = 80.0 nM 7.10
CHEMBL4068180 IC50 = 82.0 nM 7.09
CHEMBL4091912 IC50 = 84.0 nM 7.08
CHEMBL4072812 IC50 = 93.0 nM 7.03
CHEMBL4098824 IC50 = 94.0 nM 7.03
CHEMBL4092917 IC50 = 95.0 nM 7.02
CHEMBL4064292 IC50 = 100.0 nM 7.00
CHEMBL4077317 IC50 = 102.0 nM 6.99
CHEMBL4080780 IC50 = 115.0 nM 6.94
CHEMBL4062266 IC50 = 121.0 nM 6.92
CHEMBL4081145 IC50 = 124.0 nM 6.91
CHEMBL4063112 IC50 = 126.0 nM 6.90
CHEMBL4075962 IC50 = 153.0 nM 6.82
CHEMBL4099171 IC50 = 248.0 nM 6.61
CHEMBL4064184 IC50 = 366.0 nM 6.44
CHEMBL4077619 IC50 = 450.0 nM 6.35
CHEMBL3356143 IC50 = 1110.0 nM 5.96
CHEMBL4067538 IC50 > 20000.0 nM -