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Assay Detail

CHEMBL4052334

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human LOXL2 expressed in human whole blood assessed as reduction of H2O2 production from oxidative deamination of DAP preincubated for 2 hrs followed by substrate addition measured every 2 mins for 1 hr by amplex red reagent-based fluorescence assay
31
Total Activities
30
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysyl oxidase homolog 2 (CHEMBL3714029)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of 4-(Aminomethyl)-6-(trifluoromethyl)-2-(phenoxy)pyridine Derivatives as Potent, Selective, and Orally Efficacious Inhibitors of the Copper-Dependent Amine Oxidase, Lysyl Oxidase-Like 2 (LOXL2).
Rowbottom MW, Bain G, Calderon I, Lasof T, Lonergan D, Lai A, Huang F, Darlington J, Prodanovich P, Santini AM, King CD, Goulet L, Shannon KE, Ma GL, Nguyen K, MacKenna DA, Evans JF, Hutchinson JH.
J Med Chem (2017) 60 : 4403 -4423
PubMed 28471663 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 5.55 6.55
IC90 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4082808 1 6.55
CHEMBL4084982 1 6.51
CHEMBL1618272 1 6.40
CHEMBL4100783 1 6.26
CHEMBL4097435 1 6.16
CHEMBL4063337 1 6.15
CHEMBL4105137 1 6.08
CHEMBL4079398 2 6.06
CHEMBL4071963 1 6.04
CHEMBL4092740 1 5.94
CHEMBL4088453 1 5.89
CHEMBL4063420 1 5.85
CHEMBL4067192 1 5.85
CHEMBL4086010 1 5.80
CHEMBL4093765 1 5.77
CHEMBL4103928 1 5.75
CHEMBL4087273 1 5.70
CHEMBL4059919 1 5.54
CHEMBL4078119 1 5.54
CHEMBL4085053 1 5.28
CHEMBL4063906 1 5.17
CHEMBL4066637 1 5.10
CHEMBL4066192 1 5.10
CHEMBL4068580 1 5.00
CHEMBL4089707 1 4.64
CHEMBL4075255 1 4.54
CHEMBL4085480 1 4.52
CHEMBL4066086 1 4.46
CHEMBL4096173 1 4.41
CHEMBL4081837 1 4.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4082808 IC50 = 280.0 nM 6.55
CHEMBL4084982 IC50 = 310.0 nM 6.51
CHEMBL1618272 IC50 = 400.0 nM 6.40
CHEMBL4100783 IC50 = 550.0 nM 6.26
CHEMBL4097435 IC50 = 690.0 nM 6.16
CHEMBL4063337 IC50 = 710.0 nM 6.15
CHEMBL4105137 IC50 = 840.0 nM 6.08
CHEMBL4079398 IC50 = 870.0 nM 6.06
CHEMBL4071963 IC50 = 910.0 nM 6.04
CHEMBL4092740 IC50 = 1150.0 nM 5.94
CHEMBL4088453 IC50 = 1300.0 nM 5.89
CHEMBL4067192 IC50 = 1400.0 nM 5.85
CHEMBL4063420 IC50 = 1400.0 nM 5.85
CHEMBL4086010 IC50 = 1600.0 nM 5.80
CHEMBL4093765 IC50 = 1700.0 nM 5.77
CHEMBL4103928 IC50 = 1800.0 nM 5.75
CHEMBL4087273 IC50 = 2000.0 nM 5.70
CHEMBL4078119 IC50 = 2900.0 nM 5.54
CHEMBL4059919 IC50 = 2900.0 nM 5.54
CHEMBL4085053 IC50 = 5200.0 nM 5.28
CHEMBL4063906 IC50 = 6700.0 nM 5.17
CHEMBL4066637 IC50 = 7900.0 nM 5.10
CHEMBL4066192 IC50 = 7900.0 nM 5.10
CHEMBL4068580 IC50 = 9900.0 nM 5.00
CHEMBL4089707 IC50 = 23000.0 nM 4.64
CHEMBL4075255 IC50 = 29000.0 nM 4.54
CHEMBL4085480 IC50 = 30000.0 nM 4.52
CHEMBL4066086 IC50 = 35000.0 nM 4.46
CHEMBL4096173 IC50 = 39000.0 nM 4.41
CHEMBL4081837 IC50 = 40000.0 nM 4.40
CHEMBL4079398 IC90 = 5400.0 nM -