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Assay Detail

CHEMBL4052479

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human GST-tagged EGFR L858R mutant cytoplasmic domain expressed in baculovirus expression system preincubated for 20 mins followed by addition of [33P]-ATP measured after 2 hrs by filter-binding method
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Trisubstituted Imidazoles with a Rigidized Hinge Binding Motif Act As Single Digit nM Inhibitors of Clinically Relevant EGFR L858R/T790M and L858R/T790M/C797S Mutants: An Example of Target Hopping.
Juchum M, Günther M, Döring E, Sievers-Engler A, Lämmerhofer M, Laufer S.
J Med Chem (2017) 60 : 4636 -4656
PubMed 28482151 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.19 8.13

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4090582 1 8.13
CHEMBL4072874 1 8.11
CHEMBL4070454 1 7.95
CHEMBL388978 STAUROSPORINE 1 7.94
CHEMBL4100759 1 7.89
CHEMBL4066951 1 7.81
CHEMBL4077917 1 7.73
CHEMBL4097627 1 7.63
CHEMBL2179119 1 7.62
CHEMBL4088971 1 7.61
CHEMBL4094438 1 7.49
CHEMBL4102179 1 7.47
CHEMBL2179128 1 6.99
CHEMBL4078920 1 6.59
CHEMBL4077228 1 6.55
CHEMBL4069229 1 6.53
CHEMBL4064224 1 6.34
CHEMBL2179124 1 6.20
CHEMBL4071494 1 5.89
CHEMBL4062617 1 5.41
CHEMBL4061829 1 -
CHEMBL4089892 1 -
CHEMBL4085781 1 -
CHEMBL4071827 1 -
CHEMBL4097718 1 -
CHEMBL4092011 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4090582 IC50 = 7.41 nM 8.13
CHEMBL4072874 IC50 = 7.8 nM 8.11
CHEMBL4070454 IC50 = 11.2 nM 7.95
CHEMBL388978 STAUROSPORINE IC50 = 11.4 nM 7.94
CHEMBL4100759 IC50 = 13.0 nM 7.89
CHEMBL4066951 IC50 = 15.4 nM 7.81
CHEMBL4077917 IC50 = 18.7 nM 7.73
CHEMBL4097627 IC50 = 23.6 nM 7.63
CHEMBL2179119 IC50 = 24.1 nM 7.62
CHEMBL4088971 IC50 = 24.4 nM 7.61
CHEMBL4094438 IC50 = 32.1 nM 7.49
CHEMBL4102179 IC50 = 34.2 nM 7.47
CHEMBL2179128 IC50 = 102.0 nM 6.99
CHEMBL4078920 IC50 = 258.0 nM 6.59
CHEMBL4077228 IC50 = 279.0 nM 6.55
CHEMBL4069229 IC50 = 297.0 nM 6.53
CHEMBL4064224 IC50 = 459.0 nM 6.34
CHEMBL2179124 IC50 = 631.0 nM 6.20
CHEMBL4071494 IC50 = 1300.0 nM 5.89
CHEMBL4062617 IC50 = 3920.0 nM 5.41
CHEMBL4061829 IC50 < 0.3 nM -
CHEMBL4089892 IC50 < 0.3 nM -
CHEMBL4085781 IC50 - -
CHEMBL4071827 IC50 < 0.3 nM -
CHEMBL4097718 IC50 - -
CHEMBL4092011 IC50 - -