Assay Detail
Binding
CHEMBL4052946
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of NIK in human HeLa cells assessed as decrease in antilymphotoxin-beta receptor antibody-stimulated p52 translocation from cytoplasm to nucleus after 5 hrs
4
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HeLa |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Mitogen-activated protein kinase kinase kinase 14 (CHEMBL5888) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure-Based Design of Tricyclic NF-κB Inducing Kinase (NIK) Inhibitors That Have High Selectivity over Phosphoinositide-3-kinase (PI3K).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 6.70 | 7.17 |
| Inhibition | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3692450 | — | — | 1 | 7.17 |
| CHEMBL4073390 | — | — | 1 | 6.70 |
| CHEMBL4098056 | — | — | 2 | 6.22 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3692450 | — | IC50 | = | 67.0 | nM | 7.17 |
| CHEMBL4073390 | — | IC50 | = | 199.0 | nM | 6.70 |
| CHEMBL4098056 | — | IC50 | = | 607.0 | nM | 6.22 |
| CHEMBL4098056 | — | Inhibition | - | % | - |