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Assay Detail

CHEMBL4053931

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant full-length N-terminal His-tagged BTK expressed in baculovirus infected Sf9 insect cells after 60 mins by ADP-Glo kinase assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of sulfonamide-substituted diphenylpyrimidines (SFA-DPPYs) as potent Bruton's tyrosine kinase (BTK) inhibitors with improved activity toward B-cell lymphoblastic leukemia.
Liu H, Qu M, Xu L, Han X, Wang C, Shu X, Yao J, Liu K, Peng J, Li Y, Ma X.
Eur J Med Chem (2017) 135 : 60 -69
PubMed 28432946 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 8.28 9.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1873475 IBRUTINIB 4.0 1 9.47
CHEMBL4071567 1 9.38
CHEMBL3301625 SPEBRUTINIB 2.0 1 9.14
CHEMBL4070521 1 9.04
CHEMBL4092347 1 8.98
CHEMBL4065460 1 8.93
CHEMBL4061898 1 8.04
CHEMBL4097689 1 7.62
CHEMBL4094611 1 7.31
CHEMBL4103300 1 7.23
CHEMBL4086899 1 7.11
CHEMBL4089960 1 7.07
CHEMBL4074077 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1873475 IBRUTINIB IC50 = 0.34 nM 9.47
CHEMBL4071567 IC50 = 0.42 nM 9.38
CHEMBL3301625 SPEBRUTINIB IC50 = 0.72 nM 9.14
CHEMBL4070521 IC50 = 0.92 nM 9.04
CHEMBL4092347 IC50 = 1.05 nM 8.98
CHEMBL4065460 IC50 = 1.18 nM 8.93
CHEMBL4061898 IC50 = 9.14 nM 8.04
CHEMBL4097689 IC50 = 23.8 nM 7.62
CHEMBL4094611 IC50 = 48.6 nM 7.31
CHEMBL4103300 IC50 = 58.5 nM 7.23
CHEMBL4086899 IC50 = 78.6 nM 7.11
CHEMBL4089960 IC50 = 85.4 nM 7.07
CHEMBL4074077 IC50 > 100.0 nM -