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Assay Detail

CHEMBL4055562

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST-tagged recombinant human full-length ZAK expressed in baculovirus infected Sf9 insect cells using MBP as substrate after 30 mins by ADP-Glo assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase kinase kinase 20 (CHEMBL3886)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure Based Design of N-(3-((1H-Pyrazolo[3,4-b]pyridin-5-yl)ethynyl)benzenesulfonamides as Selective Leucine-Zipper and Sterile-α Motif Kinase (ZAK) Inhibitors.
Chang Y, Lu X, Shibu MA, Dai YB, Luo J, Zhang Y, Li Y, Zhao P, Zhang Z, Xu Y, Tu ZC, Zhang QW, Yun CH, Huang CY, Ding K.
J Med Chem (2017) 60 : 5927 -5932
PubMed 28586211 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.78 8.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4098896 1 8.48
CHEMBL4096439 1 8.38
CHEMBL4080860 1 8.30
CHEMBL4104859 1 8.29
CHEMBL4091071 1 8.29
CHEMBL4079935 1 8.12
CHEMBL4088739 1 8.02
CHEMBL4104171 1 7.92
CHEMBL4069195 1 7.78
CHEMBL4080626 1 7.72
CHEMBL4060505 1 7.65
CHEMBL1229517 VEMURAFENIB 4.0 1 7.50
CHEMBL4087178 1 7.37
CHEMBL4078038 1 6.84
CHEMBL4085336 1 6.00
CHEMBL4067092 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4098896 IC50 = 3.3 nM 8.48
CHEMBL4096439 IC50 = 4.2 nM 8.38
CHEMBL4080860 IC50 = 5.0 nM 8.30
CHEMBL4104859 IC50 = 5.1 nM 8.29
CHEMBL4091071 IC50 = 5.1 nM 8.29
CHEMBL4079935 IC50 = 7.5 nM 8.12
CHEMBL4088739 IC50 = 9.6 nM 8.02
CHEMBL4104171 IC50 = 12.1 nM 7.92
CHEMBL4069195 IC50 = 16.8 nM 7.78
CHEMBL4080626 IC50 = 19.2 nM 7.72
CHEMBL4060505 IC50 = 22.6 nM 7.65
CHEMBL1229517 VEMURAFENIB IC50 = 31.4 nM 7.50
CHEMBL4087178 IC50 = 42.7 nM 7.37
CHEMBL4078038 IC50 = 143.7 nM 6.84
CHEMBL4085336 IC50 = 994.1 nM 6.00
CHEMBL4067092 IC50 > 30000.0 nM -