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Assay Detail

CHEMBL4057947

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant full-length His6-tagged BTK (unknown origin) expressed in baculovirus infected Sf9 cells using biotinylated A5 peptide as substrate preincubated for 60 mins followed by substrate addition measured after 120 mins by LANCE TR-FRET assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 3-morpholino-imidazole[1,5-a]pyrazine BTK inhibitors for rheumatoid arthritis.
Boga SB, Alhassan AB, Liu J, Guiadeen D, Krikorian A, Gao X, Wang J, Yu Y, Anand R, Liu S, Yang C, Wu H, Cai J, Zhu H, Desai J, Maloney K, Gao YD, Fischmann TO, Presland J, Mansueto M, Xu Z, Leccese E, Knemeyer I, Garlisi CG, Bays N, Stivers P, Brandish PE, Hicks A, Cooper A, Kim RM, Kozlowski JA.
Bioorg Med Chem Lett (2017) 27 : 3939 -3943
PubMed 28720503 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 8.73 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4079820 1 10.00
CHEMBL4076024 1 9.70
CHEMBL4069387 1 9.60
CHEMBL4094125 1 9.52
CHEMBL4081661 1 9.52
CHEMBL4062498 1 9.43
CHEMBL4096669 1 9.40
CHEMBL4075954 1 9.40
CHEMBL4065907 1 9.40
CHEMBL4060757 1 9.23
CHEMBL4059723 1 9.15
CHEMBL4077588 1 9.10
CHEMBL4075605 1 9.05
CHEMBL4104254 1 8.92
CHEMBL4097247 1 8.48
CHEMBL4078497 1 8.47
CHEMBL4099975 1 8.28
CHEMBL4086348 1 8.23
CHEMBL4079225 1 8.18
CHEMBL4096526 1 7.97
CHEMBL4066585 1 6.88
CHEMBL4104951 1 6.53
CHEMBL4087095 1 6.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4079820 IC50 = 0.1 nM 10.00
CHEMBL4076024 IC50 = 0.2 nM 9.70
CHEMBL4069387 IC50 = 0.25 nM 9.60
CHEMBL4094125 IC50 = 0.3 nM 9.52
CHEMBL4081661 IC50 = 0.3 nM 9.52
CHEMBL4062498 IC50 = 0.37 nM 9.43
CHEMBL4096669 IC50 = 0.4 nM 9.40
CHEMBL4075954 IC50 = 0.4 nM 9.40
CHEMBL4065907 IC50 = 0.4 nM 9.40
CHEMBL4060757 IC50 = 0.59 nM 9.23
CHEMBL4059723 IC50 = 0.7 nM 9.15
CHEMBL4077588 IC50 = 0.8 nM 9.10
CHEMBL4075605 IC50 = 0.9 nM 9.05
CHEMBL4104254 IC50 = 1.2 nM 8.92
CHEMBL4097247 IC50 = 3.3 nM 8.48
CHEMBL4078497 IC50 = 3.4 nM 8.47
CHEMBL4099975 IC50 = 5.3 nM 8.28
CHEMBL4086348 IC50 = 5.9 nM 8.23
CHEMBL4079225 IC50 = 6.6 nM 8.18
CHEMBL4096526 IC50 = 10.7 nM 7.97
CHEMBL4066585 IC50 = 132.0 nM 6.88
CHEMBL4104951 IC50 = 294.0 nM 6.53
CHEMBL4087095 IC50 = 377.0 nM 6.42