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Assay Detail

CHEMBL4122817

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIF-1alpha/p300/CBP (unknown origin) transcriptional activity expressed in human HeLa cells expressing HRE-Luc/CMV-Luc after 12 hrs under hypoxic condition by dual luciferase reporter gene assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 5 — Multiple protein complex / RNA
Curated By Intermediate

Target

HIF1A/p300/CREB-binding protein (CHEMBL4296108)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Discovery of bicyclo[3,3,1]non-2-ene as a novel skeleton for HIF-1 inhibitors.
Ueda H, Yoshimori A, Nakamura H.
Bioorg Med Chem (2018) 26 : 3345 -3351
PubMed 29748146 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 5.20 5.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL333985 1 5.89
CHEMBL4126974 1 5.52
CHEMBL4128348 1 5.20
CHEMBL4129341 1 4.19
CHEMBL4125744 1 -
CHEMBL4127113 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL333985 IC50 = 1300.0 nM 5.89
CHEMBL4126974 IC50 = 3000.0 nM 5.52
CHEMBL4128348 IC50 = 6300.0 nM 5.20
CHEMBL4129341 IC50 = 65000.0 nM 4.19
CHEMBL4125744 IC50 > 100000.0 nM -
CHEMBL4127113 IC50 > 100000.0 nM -