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Assay Detail

CHEMBL4124177

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human JAK3 (781 to 1124 residues) expressed in baculovirus expression system assessed as reduction in polypeptide substrate phosphorylation in presence of ATP by ELISA
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK3 (CHEMBL2148)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development, Optimization, and Structure-Activity Relationships of Covalent-Reversible JAK3 Inhibitors Based on a Tricyclic Imidazo[5,4- d]pyrrolo[2,3- b]pyridine Scaffold.
Forster M, Chaikuad A, Dimitrov T, Döring E, Holstein J, Berger BT, Gehringer M, Ghoreschi K, Müller S, Knapp S, Laufer SA.
J Med Chem (2018) 61 : 5350 -5366
PubMed 29852068 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 7.00 8.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL221959 TOFACITINIB 4.0 1 8.46
CHEMBL4125719 1 8.40
CHEMBL4128181 1 8.05
CHEMBL1650951 1 7.96
CHEMBL4127091 1 7.96
CHEMBL4125861 1 7.85
CHEMBL4128324 1 7.64
CHEMBL4128266 1 7.57
CHEMBL4126944 1 7.52
CHEMBL4128410 1 7.29
CHEMBL4126097 1 7.23
CHEMBL4129249 1 6.89
CHEMBL4127590 1 6.89
CHEMBL4129641 1 6.87
CHEMBL4126514 1 6.74
CHEMBL4129559 1 6.69
CHEMBL4127174 1 6.65
CHEMBL4129159 1 6.43
CHEMBL4127032 1 6.41
CHEMBL4128579 1 6.36
CHEMBL4125935 1 6.33
CHEMBL4128719 1 6.31
CHEMBL4129943 1 6.30
CHEMBL4127332 1 6.30
CHEMBL4125798 1 6.26
CHEMBL4128988 1 6.24
CHEMBL4127480 1 6.19
CHEMBL4129794 1 6.17
CHEMBL4128005 1 -
CHEMBL4129808 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL221959 TOFACITINIB IC50 = 3.5 nM 8.46
CHEMBL4125719 IC50 = 4.0 nM 8.40
CHEMBL4128181 IC50 = 9.0 nM 8.05
CHEMBL1650951 IC50 = 11.0 nM 7.96
CHEMBL4127091 IC50 = 11.0 nM 7.96
CHEMBL4125861 IC50 = 14.0 nM 7.85
CHEMBL4128324 IC50 = 23.0 nM 7.64
CHEMBL4128266 IC50 = 27.0 nM 7.57
CHEMBL4126944 IC50 = 30.0 nM 7.52
CHEMBL4128410 IC50 = 51.0 nM 7.29
CHEMBL4126097 IC50 = 59.0 nM 7.23
CHEMBL4129249 IC50 = 129.0 nM 6.89
CHEMBL4127590 IC50 = 129.0 nM 6.89
CHEMBL4129641 IC50 = 135.0 nM 6.87
CHEMBL4126514 IC50 = 181.0 nM 6.74
CHEMBL4129559 IC50 = 205.0 nM 6.69
CHEMBL4127174 IC50 = 226.0 nM 6.65
CHEMBL4129159 IC50 = 368.0 nM 6.43
CHEMBL4127032 IC50 = 390.0 nM 6.41
CHEMBL4128579 IC50 = 436.0 nM 6.36
CHEMBL4125935 IC50 = 470.0 nM 6.33
CHEMBL4128719 IC50 = 490.0 nM 6.31
CHEMBL4129943 IC50 = 505.0 nM 6.30
CHEMBL4127332 IC50 = 499.0 nM 6.30
CHEMBL4125798 IC50 = 548.0 nM 6.26
CHEMBL4128988 IC50 = 571.0 nM 6.24
CHEMBL4127480 IC50 = 642.0 nM 6.19
CHEMBL4129794 IC50 = 678.0 nM 6.17
CHEMBL4128005 IC50 > 1000.0 nM -
CHEMBL4129808 IC50 > 10000.0 nM -