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Assay Detail

CHEMBL4124224

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PI3Kgamma in mouse RAW264 cells assessed as reduction in Akt phosphorylation at Ser473 residue preincubated for 15 mins followed by C5a stimulation measured after 3 mins by TR-FRET assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of Highly Isoform Selective Orally Bioavailable Phosphoinositide 3-Kinase (PI3K)-γ Inhibitors.
Pemberton N, Mogemark M, Arlbrandt S, Bold P, Cox RJ, Gardelli C, Holden NS, Karabelas K, Karlsson J, Lever S, Li X, Lindmark H, Norberg M, Perry MWD, Petersen J, Rodrigo Blomqvist S, Thomas M, Tyrchan C, Westin Eriksson A, Zlatoidsky P, Öster L.
J Med Chem (2018) 61 : 5435 -5441
PubMed 29852070 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.37 8.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4127396 1 8.60
CHEMBL4129600 1 8.30
CHEMBL4126601 1 8.20
CHEMBL4126445 1 8.20
CHEMBL4126010 1 8.20
CHEMBL4126156 1 8.10
CHEMBL4128537 1 7.70
CHEMBL4128230 1 7.70
CHEMBL4129877 1 7.50
CHEMBL4127833 1 7.40
CHEMBL4129457 1 6.60
CHEMBL4128652 1 6.40
CHEMBL4130021 1 6.40
CHEMBL4127414 1 6.20
CHEMBL4128809 1 5.10
CHEMBL4127150 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4127396 IC50 = 2.512 nM 8.60
CHEMBL4129600 IC50 = 5.012 nM 8.30
CHEMBL4126601 IC50 = 6.31 nM 8.20
CHEMBL4126445 IC50 = 6.31 nM 8.20
CHEMBL4126010 IC50 = 6.31 nM 8.20
CHEMBL4126156 IC50 = 7.943 nM 8.10
CHEMBL4128537 IC50 = 19.95 nM 7.70
CHEMBL4128230 IC50 = 19.95 nM 7.70
CHEMBL4129877 IC50 = 31.62 nM 7.50
CHEMBL4127833 IC50 = 39.81 nM 7.40
CHEMBL4129457 IC50 = 251.19 nM 6.60
CHEMBL4128652 IC50 = 398.11 nM 6.40
CHEMBL4130021 IC50 = 398.11 nM 6.40
CHEMBL4127414 IC50 = 630.96 nM 6.20
CHEMBL4128809 IC50 = 7943.28 nM 5.10
CHEMBL4127150 IC50 > 1000.0 nM -