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Assay Detail

CHEMBL4124263

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS induced nitric oxide production preincubated for 2 hrs followed by LPS challenge measured after 24 hrs by Griess reagent based assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Intermediate

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Hexahydrobenzophenanthridine alkaloids from Corydalis bungeana Turcz. and their anti-inflammatory activity.
Gao C, Du Y, Wang X, Cao H, Lin B, Liu Y, Di X.
Bioorg Med Chem Lett (2018) 28 : 2265 -2269
PubMed 29853329 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 5.35 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4130075 1 6.00
CHEMBL1209678 CHEILANTHIFOLINE 1 5.73
CHEMBL4126232 1 5.69
CHEMBL4127605 1 5.66
CHEMBL4129364 1 5.65
CHEMBL4126384 1 5.60
CHEMBL4128383 1 5.55
CHEMBL4129674 1 4.64
CHEMBL1434 MINOCYCLINE 4.0 1 4.50
CHEMBL4127010 1 4.47

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4130075 IC50 = 1000.0 nM 6.00
CHEMBL1209678 CHEILANTHIFOLINE IC50 = 1880.0 nM 5.73
CHEMBL4126232 IC50 = 2030.0 nM 5.69
CHEMBL4127605 IC50 = 2190.0 nM 5.66
CHEMBL4129364 IC50 = 2260.0 nM 5.65
CHEMBL4126384 IC50 = 2510.0 nM 5.60
CHEMBL4128383 IC50 = 2790.0 nM 5.55
CHEMBL4129674 IC50 = 23100.0 nM 4.64
CHEMBL1434 MINOCYCLINE IC50 = 31280.0 nM 4.50
CHEMBL4127010 IC50 = 33800.0 nM 4.47