Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4124447

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIF-PHD2 (181 to 426 residues) (unknown origin) using HIF1-alpha (556 to 574 residues) as substrate in presence of 2-OG preincubated for 30 mins followed by OPD addition measured after 10 mins by fluorescence assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Egl nine homolog 1 (CHEMBL5697)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Click Chemistry-Based Discovery of [3-Hydroxy-5-(1 H-1,2,3-triazol-4-yl)picolinoyl]glycines as Orally Active Hypoxia-Inducing Factor Prolyl Hydroxylase Inhibitors with Favorable Safety Profiles for the Treatment of Anemia.
Wu Y, Jiang Z, Li Z, Gu J, You Q, Zhang X.
J Med Chem (2018) 61 : 5332 -5349
PubMed 29856623 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.26 5.88

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4126434 1 5.88
CHEMBL4129555 1 5.56
CHEMBL4128320 1 5.27
CHEMBL2338329 ROXADUSTAT 4.0 1 5.24
CHEMBL4125856 1 5.21
CHEMBL4126256 1 5.19
CHEMBL4127232 1 5.16
CHEMBL4129725 1 5.09
CHEMBL4129131 1 4.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4126434 IC50 = 1310.0 nM 5.88
CHEMBL4129555 IC50 = 2750.0 nM 5.56
CHEMBL4128320 IC50 = 5340.0 nM 5.27
CHEMBL2338329 ROXADUSTAT IC50 = 5740.0 nM 5.24
CHEMBL4125856 IC50 = 6110.0 nM 5.21
CHEMBL4126256 IC50 = 6430.0 nM 5.19
CHEMBL4127232 IC50 = 6880.0 nM 5.16
CHEMBL4129725 IC50 = 8130.0 nM 5.09
CHEMBL4129131 IC50 = 17060.0 nM 4.77