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Assay Detail

CHEMBL4124698

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to 80S ribosome in human HuH7 cells assessed as inhibition of PCSK9 mRNA translation after overnight incubation by ELISA
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Huh-7
Confidence 7 — Homologous single protein target
Curated By Intermediate

Target

80S Ribosome (CHEMBL3987582)
Type PROTEIN NUCLEIC-ACID COMPLEX
Organism Homo sapiens

Publication

Small Molecule Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Inhibitors: Hit to Lead Optimization of Systemic Agents.
Londregan AT, Wei L, Xiao J, Lintner NG, Petersen D, Dullea RG, McClure KF, Bolt MW, Warmus JS, Coffey SB, Limberakis C, Genovino J, Thuma BA, Hesp KD, Aspnes GE, Reidich B, Salatto CT, Chabot JR, Cate JHD, Liras S, Piotrowski DW.
J Med Chem (2018) 61 : 5704 -5718
PubMed 29878763 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.66 6.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4114159 1 6.11
CHEMBL4109308 1 6.09
CHEMBL4126894 1 6.05
CHEMBL4126496 1 6.05
CHEMBL4130157 1 5.89
CHEMBL4107559 1 5.86
CHEMBL4108338 1 5.84
CHEMBL4128388 1 5.62
CHEMBL4127458 1 5.55
CHEMBL4127311 1 5.53
CHEMBL4126072 1 5.51
CHEMBL4128250 1 5.08
CHEMBL4127016 1 5.03
CHEMBL4128560 1 5.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4114159 IC50 = 780.0 nM 6.11
CHEMBL4109308 IC50 = 820.0 nM 6.09
CHEMBL4126894 IC50 = 900.0 nM 6.05
CHEMBL4126496 IC50 = 900.0 nM 6.05
CHEMBL4130157 IC50 = 1290.0 nM 5.89
CHEMBL4107559 IC50 = 1370.0 nM 5.86
CHEMBL4108338 IC50 = 1440.0 nM 5.84
CHEMBL4128388 IC50 = 2380.0 nM 5.62
CHEMBL4127458 IC50 = 2820.0 nM 5.55
CHEMBL4127311 IC50 = 2970.0 nM 5.53
CHEMBL4126072 IC50 = 3080.0 nM 5.51
CHEMBL4128250 IC50 = 8370.0 nM 5.08
CHEMBL4127016 IC50 = 9320.0 nM 5.03
CHEMBL4128560 IC50 = 9240.0 nM 5.03