Assay Detail
Binding
CHEMBL4132839
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of TS/AICARFTase/GARFTase in human KB cells assessed as reduction in cell proliferation in folate free medium after 72 hrs in presence of leucovorin by MTT assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 5 — Multiple protein complex / RNA |
| Curated By | Intermediate |
Target
Thymidylate synthase/GAR transformylase/AICAR transformylase (CHEMBL3885528) ↗| Type | PROTEIN FAMILY |
| Organism | Homo sapiens |
Publication
Novel 6-substituted benzoyl and non-benzoyl straight chain pyrrolo[2,3-d]pyrimidines as potential antitumor agents with multitargeted inhibition of TS, GARFTase and AICARFTase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 8.08 | 10.11 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4177183 | — | — | 1 | 10.11 |
| CHEMBL4162649 | — | — | 1 | 9.85 |
| CHEMBL4170578 | — | — | 1 | 8.12 |
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 8.00 |
| CHEMBL4174018 | — | — | 1 | 7.37 |
| CHEMBL225072 | PEMETREXED | 4.0 | 1 | 7.16 |
| CHEMBL4163732 | — | — | 1 | 7.04 |
| CHEMBL4166099 | — | — | 1 | 6.97 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4177183 | — | IC50 | = | 0.078 | nM | 10.11 |
| CHEMBL4162649 | — | IC50 | = | 0.14 | nM | 9.85 |
| CHEMBL4170578 | — | IC50 | = | 7.5 | nM | 8.12 |
| CHEMBL34259 | METHOTREXATE | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL4174018 | — | IC50 | = | 43.0 | nM | 7.37 |
| CHEMBL225072 | PEMETREXED | IC50 | = | 70.0 | nM | 7.16 |
| CHEMBL4163732 | — | IC50 | = | 92.0 | nM | 7.04 |
| CHEMBL4166099 | — | IC50 | = | 107.0 | nM | 6.97 |