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Assay Detail

CHEMBL4132963

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABCC1 in human H69AR cells assessed as reduction in calcein-AM efflux preincubated for 30 mins followed by calcein-AM addition measured at 60 secs interval for 60 mins by fluorescence assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Multidrug resistance-associated protein 1 (CHEMBL3004)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological investigation of 2,4-substituted quinazolines as highly potent inhibitors of breast cancer resistance protein (ABCG2).
Krapf MK, Gallus J, Wiese M.
Eur J Med Chem (2017) 139 : 587 -611
PubMed 28841513 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 4.83 5.45

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL160 CYCLOSPORINE 4.0 1 5.45
CHEMBL4159635 1 5.11
CHEMBL3092486 1 4.39
CHEMBL4160529 1 4.36
CHEMBL4172917 1 -
CHEMBL4161421 1 -
CHEMBL4171304 1 -
CHEMBL4163917 1 -
CHEMBL4171781 1 -
CHEMBL4161988 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL160 CYCLOSPORINE IC50 = 3530.0 nM 5.45
CHEMBL4159635 IC50 = 7720.0 nM 5.11
CHEMBL3092486 IC50 = 40600.0 nM 4.39
CHEMBL4160529 IC50 = 44200.0 nM 4.36
CHEMBL4172917 IC50 - -
CHEMBL4161421 IC50 - -
CHEMBL4171304 IC50 - -
CHEMBL4163917 IC50 - -
CHEMBL4171781 IC50 - -
CHEMBL4161988 IC50 - -