Assay Detail
Binding
CHEMBL4133556
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human AKR1C3 expressed in Escherichia coli BL21 (DE) using [14C]androstenedione as substrate in presence of NADPH generating system
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Aldo-keto reductase family 1 member C3 (CHEMBL4681) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Hydroxytriazole derivatives as potent and selective aldo-keto reductase 1C3 (AKR1C3) inhibitors discovered by bioisosteric scaffold hopping approach.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 4.97 | 5.45 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4174786 | — | — | 1 | 5.45 |
| CHEMBL23588 | FLUFENAMIC ACID | 2.0 | 1 | 5.06 |
| CHEMBL4166887 | — | — | 1 | 4.99 |
| CHEMBL4159654 | — | — | 1 | 4.88 |
| CHEMBL4163031 | — | — | 1 | 4.48 |
| CHEMBL4177097 | — | — | 1 | - |
| CHEMBL4166474 | — | — | 1 | - |
| CHEMBL4173733 | — | — | 1 | - |
| CHEMBL4170286 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4174786 | — | IC50 | = | 3560.0 | nM | 5.45 |
| CHEMBL23588 | FLUFENAMIC ACID | IC50 | = | 8630.0 | nM | 5.06 |
| CHEMBL4166887 | — | IC50 | = | 10190.0 | nM | 4.99 |
| CHEMBL4159654 | — | IC50 | = | 13220.0 | nM | 4.88 |
| CHEMBL4163031 | — | IC50 | = | 32790.0 | nM | 4.48 |
| CHEMBL4177097 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL4166474 | — | IC50 | >= | 50000.0 | nM | - |
| CHEMBL4173733 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL4170286 | — | IC50 | > | 50000.0 | nM | - |