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Assay Detail

CHEMBL4136450

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST-tagged c-MET cytoplasmic domain (956 to 1390 residues) expressed in baculovirus expression system using peptide substrate after 1 hr by mobility shift assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hepatocyte growth factor receptor (CHEMBL3717)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of novel 2,4-diarylaminopyrimidine analogues as ALK and ROS1 dual inhibitors to overcome crizotinib-resistant mutants including G1202R.
Wang Y, Chen S, Hu G, Wang J, Gou W, Zuo D, Gu Y, Gong P, Zhai X.
Eur J Med Chem (2018) 143 : 123 -136
PubMed 29174809 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.58 8.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL601719 CRIZOTINIB 4.0 1 8.49
CHEMBL4168473 1 6.15
CHEMBL4166436 1 6.14
CHEMBL4167072 1 6.09
CHEMBL4159161 1 6.01
CHEMBL4163707 1 -
CHEMBL4160581 1 -
CHEMBL4174347 1 -
CHEMBL4166038 1 -
CHEMBL2403108 CERITINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL601719 CRIZOTINIB IC50 = 3.2 nM 8.49
CHEMBL4168473 IC50 = 703.0 nM 6.15
CHEMBL4166436 IC50 = 731.0 nM 6.14
CHEMBL4167072 IC50 = 820.0 nM 6.09
CHEMBL4159161 IC50 = 967.0 nM 6.01
CHEMBL4163707 IC50 > 1000.0 nM -
CHEMBL4160581 IC50 > 1000.0 nM -
CHEMBL4174347 IC50 > 1000.0 nM -
CHEMBL4166038 IC50 > 1000.0 nM -
CHEMBL2403108 CERITINIB IC50 > 1000.0 nM -