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Assay Detail

CHEMBL4136686

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Negative allosteric modulation of rat mGlu7 expressed in HEK cells co-expressing Galpha15 assessed as reduction in L-AP4-induced intracellular calcium flux pretreated for 142 secs followed by glutamate addition and subsequent stimulation of L-AP4 for 40 secs by Fluo-4 AM dye based fluorescence assay
24
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 7 (CHEMBL3879)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

VU6010608, a Novel mGlu7 NAM from a Series of N-(2-(1H-1,2,4-Triazol-1-yl)-5-(trifluoromethoxy)phenyl)benzamides.
Reed CW, McGowan KM, Spearing PK, Stansley BJ, Roenfanz HF, Engers DW, Rodriguez AL, Engelberg EM, Luscombe VB, Loch MT, Remke DH, Rook JM, Blobaum AL, Conn PJ, Niswender CM, Lindsley CW.
ACS Med Chem Lett (2017) 8 : 1326 -1330
PubMed 29259756 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.01 6.29
Activity 8 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4169114 3 6.29
CHEMBL4174742 3 6.17
CHEMBL4160395 3 6.15
CHEMBL4176971 3 6.12
CHEMBL4161847 3 6.11
CHEMBL4165849 3 6.11
CHEMBL4170997 3 5.87
CHEMBL4160779 3 5.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4169114 IC50 = 512.86 nM 6.29
CHEMBL4169114 IC50 = 510.0 nM 6.29
CHEMBL4174742 IC50 = 676.08 nM 6.17
CHEMBL4174742 IC50 = 676.0 nM 6.17
CHEMBL4160395 IC50 = 707.95 nM 6.15
CHEMBL4160395 IC50 = 710.0 nM 6.15
CHEMBL4176971 IC50 = 760.0 nM 6.12
CHEMBL4176971 IC50 = 758.58 nM 6.12
CHEMBL4161847 IC50 = 780.0 nM 6.11
CHEMBL4161847 IC50 = 776.25 nM 6.11
CHEMBL4165849 IC50 = 780.0 nM 6.11
CHEMBL4165849 IC50 = 776.25 nM 6.11
CHEMBL4170997 IC50 = 1348.96 nM 5.87
CHEMBL4170997 IC50 = 1400.0 nM 5.85
CHEMBL4160779 IC50 = 5800.0 nM 5.24
CHEMBL4160779 IC50 = 5754.4 nM 5.24
CHEMBL4160395 Activity = 11.0 % -
CHEMBL4165849 Activity = 14.0 % -
CHEMBL4161847 Activity = 19.0 % -
CHEMBL4170997 Activity = 10.0 % -
CHEMBL4169114 Activity = 11.0 % -
CHEMBL4176971 Activity = 15.0 % -
CHEMBL4174742 Activity = 8.0 % -
CHEMBL4160779 Activity = 19.0 % -