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Assay Detail

CHEMBL4141610

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Binding
Inhibition of FITC-labeled Nrf2 binding to human Keap1 Kelch domain (322 to 609 residues) expressed in Escherichia coli (DE3) after 30 mins by fluorescence polarization assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kelch-like ECH-associated protein 1 (CHEMBL2069156)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a head-to-tail cyclic peptide as the Keap1-Nrf2 protein-protein interaction inhibitor with high cell potency.
Lu MC, Jiao Q, Liu T, Tan SJ, Zhou HS, You QD, Jiang ZY.
Eur J Med Chem (2018) 143 : 1578 -1589
PubMed 29117896 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.47 7.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4169002 1 7.74
CHEMBL4174405 1 7.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4169002 IC50 = 18.31 nM 7.74
CHEMBL4174405 IC50 = 63.15 nM 7.20