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Assay Detail

CHEMBL4143891

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at recombinant rat TRPM8 expressed in HEK293 cells assessed as inhibition of icilin-induced intracellular calcium level incubated for 5 mins followed by agonist stimulation by Fluo4-AM probe based fluorescence assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Iodine-Promoted Aromatization of p-Menthane-Type Phytocannabinoids.
Pollastro F, Caprioglio D, Marotta P, Moriello AS, De Petrocellis L, Taglialatela-Scafati O, Appendino G.
J Nat Prod (2018) 81 : 630 -633
PubMed 29240420 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.49 6.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL74415 CANNABINOL 3.0 1 6.68
CHEMBL4170268 1 6.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL74415 CANNABINOL IC50 = 210.0 nM 6.68
CHEMBL4170268 IC50 = 500.0 nM 6.30