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Assay Detail

CHEMBL4144031

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human His-tagged Brd2 bromodomain 1 expressed in Escherichia coli BL21 (DE3) preincubated for 30 mins followed by addition of biotinylated acetyl-histone H4 peptide and measured after 1 hr by AlphaScreen assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 2 (CHEMBL1293289)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Biological Activity of 1,2,3-Triazolobenzodiazepine BET Bromodomain Inhibitors.
Sharp PP, Garnier JM, Hatfaludi T, Xu Z, Segal D, Jarman KE, Jousset H, Garnham A, Feutrill JT, Cuzzupe A, Hall P, Taylor S, Walkley CR, Tyler D, Dawson MA, Czabotar P, Wilks AF, Glaser S, Huang DCS, Burns CJ.
ACS Med Chem Lett (2017) 8 : 1298 -1303
PubMed 29259751 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 7.10 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4165389 1 8.22
CHEMBL2132936 1 7.58
CHEMBL4168815 1 7.48
CHEMBL4161767 1 7.47
CHEMBL4177080 1 7.46
CHEMBL4170756 1 7.42
CHEMBL4167362 1 7.36
CHEMBL4162832 1 7.29
CHEMBL4159455 1 7.28
CHEMBL4173305 1 7.28
CHEMBL4174587 1 7.27
CHEMBL4173105 1 7.25
CHEMBL4176672 1 7.19
CHEMBL4176902 1 7.17
CHEMBL4169698 1 7.10
CHEMBL4173537 1 7.02
CHEMBL4174764 1 6.94
CHEMBL4169877 1 6.89
CHEMBL4174918 1 6.89
CHEMBL4166286 1 6.87
CHEMBL4166671 1 6.82
CHEMBL4166861 1 6.73
CHEMBL4165200 1 6.69
CHEMBL4164698 1 6.58
CHEMBL4161952 1 6.56
CHEMBL4162818 1 6.47
CHEMBL4166455 1 6.41
CHEMBL4167034 1 -
CHEMBL4160537 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4165389 IC50 = 6.0 nM 8.22
CHEMBL2132936 IC50 = 26.1 nM 7.58
CHEMBL4168815 IC50 = 33.0 nM 7.48
CHEMBL4161767 IC50 = 34.0 nM 7.47
CHEMBL4177080 IC50 = 35.0 nM 7.46
CHEMBL4170756 IC50 = 38.0 nM 7.42
CHEMBL4167362 IC50 = 44.0 nM 7.36
CHEMBL4162832 IC50 = 51.0 nM 7.29
CHEMBL4159455 IC50 = 53.0 nM 7.28
CHEMBL4173305 IC50 = 53.0 nM 7.28
CHEMBL4174587 IC50 = 54.0 nM 7.27
CHEMBL4173105 IC50 = 56.0 nM 7.25
CHEMBL4176672 IC50 = 64.0 nM 7.19
CHEMBL4176902 IC50 = 68.0 nM 7.17
CHEMBL4169698 IC50 = 79.0 nM 7.10
CHEMBL4173537 IC50 = 95.0 nM 7.02
CHEMBL4174764 IC50 = 116.0 nM 6.94
CHEMBL4169877 IC50 = 129.0 nM 6.89
CHEMBL4174918 IC50 = 129.0 nM 6.89
CHEMBL4166286 IC50 = 136.0 nM 6.87
CHEMBL4166671 IC50 = 151.0 nM 6.82
CHEMBL4166861 IC50 = 185.0 nM 6.73
CHEMBL4165200 IC50 = 204.0 nM 6.69
CHEMBL4164698 IC50 = 263.5 nM 6.58
CHEMBL4161952 IC50 = 273.0 nM 6.56
CHEMBL4162818 IC50 = 340.0 nM 6.47
CHEMBL4166455 IC50 = 388.0 nM 6.41
CHEMBL4167034 IC50 > 999.0 nM -
CHEMBL4160537 IC50 > 10000.0 nM -