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Assay Detail

CHEMBL4145343

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to DNA-tagged human GAK expressed in Escherichia coli BL21 using immobilised ligand incubated for 1 hr by quantitative PCR
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cyclin-G-associated kinase (CHEMBL4355)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of Isothiazolo[4,3- b]pyridine-Based Inhibitors of Cyclin G Associated Kinase (GAK) with Broad-Spectrum Antiviral Activity.
Pu SY, Wouters R, Schor S, Rozenski J, Barouch-Bentov R, Prugar LI, O'Brien CM, Brannan JM, Dye JM, Herdewijn P, De Jonghe S, Einav S.
J Med Chem (2018) 61 : 6178 -6192
PubMed 29953812 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 22 6.95 8.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL553 ERLOTINIB 4.0 1 8.51
CHEMBL3425867 1 8.05
CHEMBL4165336 1 7.96
CHEMBL4169166 1 7.75
CHEMBL4163357 1 7.72
CHEMBL4174075 1 7.58
CHEMBL4177020 1 7.46
CHEMBL4159994 1 7.34
CHEMBL4171678 1 7.28
CHEMBL4170643 1 7.21
CHEMBL4163801 1 7.10
CHEMBL4172550 1 7.05
CHEMBL4160425 1 6.80
CHEMBL4175117 1 6.77
CHEMBL4167255 1 6.72
CHEMBL4168337 1 6.68
CHEMBL4159403 1 6.60
CHEMBL4161901 1 6.10
CHEMBL4166209 1 6.01
CHEMBL4162766 1 5.80
CHEMBL4170698 1 5.37
CHEMBL4167311 1 5.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL553 ERLOTINIB Kd = 3.1 nM 8.51
CHEMBL3425867 Kd = 8.9 nM 8.05
CHEMBL4165336 Kd = 11.0 nM 7.96
CHEMBL4169166 Kd = 18.0 nM 7.75
CHEMBL4163357 Kd = 19.0 nM 7.72
CHEMBL4174075 Kd = 26.0 nM 7.58
CHEMBL4177020 Kd = 35.0 nM 7.46
CHEMBL4159994 Kd = 46.0 nM 7.34
CHEMBL4171678 Kd = 52.0 nM 7.28
CHEMBL4170643 Kd = 61.0 nM 7.21
CHEMBL4163801 Kd = 80.0 nM 7.10
CHEMBL4172550 Kd = 89.0 nM 7.05
CHEMBL4160425 Kd = 160.0 nM 6.80
CHEMBL4175117 Kd = 170.0 nM 6.77
CHEMBL4167255 Kd = 190.0 nM 6.72
CHEMBL4168337 Kd = 210.0 nM 6.68
CHEMBL4159403 Kd = 250.0 nM 6.60
CHEMBL4161901 Kd = 800.0 nM 6.10
CHEMBL4166209 Kd = 970.0 nM 6.01
CHEMBL4162766 Kd = 1600.0 nM 5.80
CHEMBL4170698 Kd = 4300.0 nM 5.37
CHEMBL4167311 Kd = 7200.0 nM 5.14