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Assay Detail

CHEMBL4149733

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at recombinant rat N-terminal FLAG/eYFP-fused GPR35 expressed in HEK293 cells assessed as induction of beta-arrestin recruitment by BRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G protein-coupled receptor GPR35 (CHEMBL2390812)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

SAR Studies of N-[2-(1H-Tetrazol-5-yl)phenyl]benzamide Derivatives as Potent G Protein-Coupled Receptor-35 Agonists.
Wei L, Hou T, Lu C, Wang J, Zhang X, Fang Y, Zhao Y, Feng J, Li J, Qu L, Piao HL, Liang X.
ACS Med Chem Lett (2018) 9 : 422 -427
PubMed 29795753 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 7.02 7.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1201198 PEMIROLAST 4.0 1 7.02

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1201198 PEMIROLAST EC50 = 95.0 nM 7.02