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Assay Detail

CHEMBL4150504

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells pretreated for 1 hr followed by LPS-stimulation and measured after 24 hrs by Griess assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Intermediate

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Chemical Constituents of Apios americana Tubers and Their Inhibitory Activities on Nitric Oxide Production in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
Seo YH, Jeon JH, Jeong M, Ryu SM, Jeon WK, Jang DS, Shim SH, Lee D, Choi JH, Lee J.
J Nat Prod (2018) 81 : 1598 -1603
PubMed 29932657 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.23 6.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4169379 1 6.42
CHEMBL4159123 1 6.38
CHEMBL4173779 1 6.33
CHEMBL593429 ALLANTOIN 2.0 1 6.30
CHEMBL4164332 1 6.22
CHEMBL4173195 1 6.22
CHEMBL4169771 1 6.15
CHEMBL7889 L-NIL 1 5.81
CHEMBL312186 1 -
CHEMBL4162791 1 -
CHEMBL4170728 1 -
CHEMBL4165175 1 -
CHEMBL4175787 1 -
CHEMBL4161741 1 -
CHEMBL4172376 1 -
CHEMBL4176874 1 -
CHEMBL486625 GENISTIN 1 -
CHEMBL1479463 1 -
CHEMBL4165584 1 -
CHEMBL6665 1 -
CHEMBL44 GENISTEIN 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4169379 IC50 = 380.0 nM 6.42
CHEMBL4159123 IC50 = 420.0 nM 6.38
CHEMBL4173779 IC50 = 470.0 nM 6.33
CHEMBL593429 ALLANTOIN IC50 = 500.0 nM 6.30
CHEMBL4164332 IC50 = 600.0 nM 6.22
CHEMBL4173195 IC50 = 600.0 nM 6.22
CHEMBL4169771 IC50 = 710.0 nM 6.15
CHEMBL7889 L-NIL IC50 = 1550.0 nM 5.81
CHEMBL312186 IC50 > 1000.0 nM -
CHEMBL4162791 IC50 > 1000.0 nM -
CHEMBL4170728 IC50 > 1000.0 nM -
CHEMBL4165175 IC50 > 1000.0 nM -
CHEMBL4175787 IC50 > 1000.0 nM -
CHEMBL4161741 IC50 > 1000.0 nM -
CHEMBL4172376 IC50 > 1000.0 nM -
CHEMBL4176874 IC50 > 1000.0 nM -
CHEMBL486625 GENISTIN IC50 > 1000.0 nM -
CHEMBL1479463 IC50 > 1000.0 nM -
CHEMBL4165584 IC50 > 1000.0 nM -
CHEMBL6665 IC50 > 1000.0 nM -
CHEMBL44 GENISTEIN IC50 > 1000.0 nM -