Assay Detail
Binding
CHEMBL4150561
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of NOTUM in human SW620 cells conditioned medium assessed as preservation of Wnt3A activity pretreated for 1 hr followed by Wnt3A producing L-cell addition for 2 hrs and subsequent HEK393-STF cell addition and measured after 24 hrs by luciferase reporter gene assay
3
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Palmitoleoyl-protein carboxylesterase NOTUM (CHEMBL3714531) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Selective Irreversible Inhibitors of the Wnt-Deacylating Enzyme NOTUM Developed by Activity-Based Protein Profiling.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 7.05 | 7.05 |
| Inhibition | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4174188 | — | — | 2 | 7.05 |
| CHEMBL4171791 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4174188 | — | IC50 | = | 89.0 | nM | 7.05 |
| CHEMBL4174188 | — | Inhibition | - | % | - | |
| CHEMBL4171791 | — | IC50 | > | 5000.0 | nM | - |