Assay Detail
Binding
CHEMBL4150563
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of rhodamine-tagged FP probe binding to NOTUM in human SW620 cells conditioned media pretreated for 30 mins followed by rhodamine-tagged FP probe addition and measured after 30 mins by SDS-PAGE fluorescence analysis
6
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Palmitoleoyl-protein carboxylesterase NOTUM (CHEMBL3714531) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Selective Irreversible Inhibitors of the Wnt-Deacylating Enzyme NOTUM Developed by Activity-Based Protein Profiling.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 7.04 | 7.89 |
| Inhibition | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4174188 | — | — | 1 | 7.89 |
| CHEMBL4163486 | — | — | 1 | 6.70 |
| CHEMBL4160119 | — | — | 1 | 6.54 |
| CHEMBL4170569 | — | — | 1 | - |
| CHEMBL4170762 | — | — | 1 | - |
| CHEMBL4171791 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4174188 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL4163486 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4160119 | — | IC50 | = | 290.0 | nM | 6.54 |
| CHEMBL4170569 | — | Inhibition | - | % | - | |
| CHEMBL4170762 | — | IC50 | > | 10000.0 | nM | - |
| CHEMBL4171791 | — | IC50 | > | 10000.0 | nM | - |