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Assay Detail

CHEMBL4151776

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal His6-tagged human full length CDK2/N-terminal GST-tagged human full length Cyclin A using histone H1 and [gamma-32P]-ATP incubated for 30 mins by phosphorimage based assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Intermediate

Target

CDK2/Cyclin A2 (CHEMBL3038469)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Development of Second-Generation CDK2 Inhibitors for the Prevention of Cisplatin-Induced Hearing Loss.
Hazlitt RA, Teitz T, Bonga JD, Fang J, Diao S, Iconaru L, Yang L, Goktug AN, Currier DG, Chen T, Rankovic Z, Min J, Zuo J.
J Med Chem (2018) 61 : 7700 -7709
PubMed 30091915 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 7.21 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL518383 1 8.52
CHEMBL456963 1 7.92
CHEMBL457177 1 7.72
CHEMBL510578 1 7.60
CHEMBL497306 1 7.46
CHEMBL507167 1 7.42
CHEMBL1187319 1 7.36
CHEMBL445813 AT-7519 2.0 1 7.33
CHEMBL463579 1 7.05
CHEMBL497477 1 6.96
CHEMBL463580 1 6.85
CHEMBL457388 1 6.85
CHEMBL448316 1 6.12
CHEMBL457208 1 5.80
CHEMBL4160645 1 -
CHEMBL4165150 1 -
CHEMBL4175760 1 -
CHEMBL4161715 1 -
CHEMBL4172348 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL518383 IC50 = 3.0 nM 8.52
CHEMBL456963 IC50 = 12.0 nM 7.92
CHEMBL457177 IC50 = 19.0 nM 7.72
CHEMBL510578 IC50 = 25.0 nM 7.60
CHEMBL497306 IC50 = 35.0 nM 7.46
CHEMBL507167 IC50 = 38.0 nM 7.42
CHEMBL1187319 IC50 = 44.0 nM 7.36
CHEMBL445813 AT-7519 IC50 = 47.0 nM 7.33
CHEMBL463579 IC50 = 90.0 nM 7.05
CHEMBL497477 IC50 = 110.0 nM 6.96
CHEMBL463580 IC50 = 140.0 nM 6.85
CHEMBL457388 IC50 = 140.0 nM 6.85
CHEMBL448316 IC50 = 750.0 nM 6.12
CHEMBL457208 IC50 = 1600.0 nM 5.80
CHEMBL4160645 IC50 = 760200.0 nM -
CHEMBL4165150 IC50 > 10000.0 nM -
CHEMBL4175760 IC50 > 10000.0 nM -
CHEMBL4161715 IC50 > 10000.0 nM -
CHEMBL4172348 IC50 > 10000.0 nM -