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Assay Detail

CHEMBL4151887

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inverse agonist activity at RORgammat in human TH17 cells assessed as inhibition of IL17 release incubated for 4 days by HTRF assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Nuclear receptor ROR-gamma (CHEMBL1741186)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potent and Orally Bioavailable Inverse Agonists of RORγt Resulting from Structure-Based Design.
Narjes F, Xue Y, von Berg S, Malmberg J, Llinas A, Olsson RI, Jirholt J, Grindebacke H, Leffler A, Hossain N, Lepistö M, Thunberg L, Leek H, Aagaard A, McPheat J, Hansson EL, Bäck E, Tångefjord S, Chen R, Xiong Y, Hongbin G, Hansson TG.
J Med Chem (2018) 61 : 7796 -7813
PubMed 30095900 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.06 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3105692 1 8.22
CHEMBL4159819 1 7.96
CHEMBL4170472 1 7.64
CHEMBL4163215 1 7.60
CHEMBL4160227 1 7.60
CHEMBL4176887 1 7.50
CHEMBL4168454 1 7.46
CHEMBL4163193 1 7.31
CHEMBL4168496 1 7.24
CHEMBL4167096 1 7.19
CHEMBL4166982 1 7.00
CHEMBL4167788 1 7.00
CHEMBL4170500 1 6.84
CHEMBL3105681 1 6.75
CHEMBL4174263 1 6.72
CHEMBL4169028 1 6.19
CHEMBL4159395 1 6.16
CHEMBL4174976 1 6.00
CHEMBL4171536 1 5.77
CHEMBL4175680 1 -
CHEMBL4161408 1 -
CHEMBL4162759 1 -
CHEMBL4173467 1 -
CHEMBL4173025 1 -
CHEMBL4161696 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3105692 IC50 = 6.0 nM 8.22
CHEMBL4159819 IC50 = 11.0 nM 7.96
CHEMBL4170472 IC50 = 23.0 nM 7.64
CHEMBL4163215 IC50 = 25.0 nM 7.60
CHEMBL4160227 IC50 = 25.0 nM 7.60
CHEMBL4176887 IC50 = 32.0 nM 7.50
CHEMBL4168454 IC50 = 35.0 nM 7.46
CHEMBL4163193 IC50 = 49.0 nM 7.31
CHEMBL4168496 IC50 = 57.0 nM 7.24
CHEMBL4167096 IC50 = 65.0 nM 7.19
CHEMBL4166982 IC50 = 100.0 nM 7.00
CHEMBL4167788 IC50 = 100.0 nM 7.00
CHEMBL4170500 IC50 = 144.0 nM 6.84
CHEMBL3105681 IC50 = 180.0 nM 6.75
CHEMBL4174263 IC50 = 190.0 nM 6.72
CHEMBL4169028 IC50 = 640.0 nM 6.19
CHEMBL4159395 IC50 = 700.0 nM 6.16
CHEMBL4174976 IC50 = 990.0 nM 6.00
CHEMBL4171536 IC50 = 1690.0 nM 5.77
CHEMBL4175680 IC50 > 3300.0 nM -
CHEMBL4161408 IC50 > 3000.0 nM -
CHEMBL4162759 IC50 - -
CHEMBL4173467 IC50 > 3300.0 nM -
CHEMBL4173025 IC50 > 3300.0 nM -
CHEMBL4161696 IC50 > 3300.0 nM -