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Assay Detail

CHEMBL4155090

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant rat DYRK1A kinase domain (1 to 499 residues) expressed in bacterial expression system using KKISGRLSPIMTEQ as substrate after 30 mins by ADP-Glo reagent based luminescence assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dual specificity tyrosine-phosphorylation-regulated kinase 1A (CHEMBL5508)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Structure-based design of novel quinoxaline-2-carboxylic acids and analogues as Pim-1 inhibitors.
Oyallon B, Brachet-Botineau M, Logé C, Bonnet P, Souab M, Robert T, Ruchaud S, Bach S, Berthelot P, Gouilleux F, Viaud-Massuard MC, Denevault-Sabourin C.
Eur J Med Chem (2018) 154 : 101 -109
PubMed 29778892 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.37 7.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4163110 1 7.01
CHEMBL4177432 1 6.57
CHEMBL4166554 1 6.13
CHEMBL4174068 1 5.78
CHEMBL4170635 1 -
CHEMBL1642 IMATINIB MESYLATE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4163110 IC50 = 98.0 nM 7.01
CHEMBL4177432 IC50 = 270.0 nM 6.57
CHEMBL4166554 IC50 = 740.0 nM 6.13
CHEMBL4174068 IC50 = 1670.0 nM 5.78
CHEMBL4170635 IC50 > 10000.0 nM -
CHEMBL1642 IMATINIB MESYLATE IC50 - -