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Assay Detail

CHEMBL4156072

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]ZM241385 from human adenosine A2A receptor expressed in CHO cell membranes measured after 60 mins by scintillation counting method
21
Total Activities
17
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationship studies and pharmacological characterization of N5-heteroarylalkyl-substituted-2-(2-furanyl)thiazolo[5,4-d]pyrimidine-5,7-diamine-based derivatives as inverse agonists at human A2A adenosine receptor.
Varano F, Catarzi D, Vincenzi F, Falsini M, Pasquini S, Borea PA, Colotta V, Varani K.
Eur J Med Chem (2018) 155 : 552 -561
PubMed 29909340 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 8.37 9.62
KiL 4 - -
KiH 4 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4162638 1 9.62
CHEMBL4159215 1 9.60
CHEMBL4167813 1 9.38
CHEMBL113142 ZM-241385 1 9.06
CHEMBL4167557 1 8.67
CHEMBL4162037 1 8.65
CHEMBL4172818 1 8.29
CHEMBL4168016 1 8.00
CHEMBL4175676 1 7.92
CHEMBL4168980 1 7.70
CHEMBL4175878 1 7.66
CHEMBL4172715 1 7.47
CHEMBL4165484 1 6.84
CHEMBL4172264 2 -
CHEMBL4167125 2 -
CHEMBL4172399 2 -
CHEMBL4161758 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4162638 Ki = 0.24 nM 9.62
CHEMBL4159215 Ki = 0.25 nM 9.60
CHEMBL4167813 Ki = 0.42 nM 9.38
CHEMBL113142 ZM-241385 Ki = 0.87 nM 9.06
CHEMBL4167557 Ki = 2.15 nM 8.67
CHEMBL4162037 Ki = 2.24 nM 8.65
CHEMBL4172818 Ki = 5.14 nM 8.29
CHEMBL4168016 Ki = 10.1 nM 8.00
CHEMBL4175676 Ki = 12.0 nM 7.92
CHEMBL4168980 Ki = 20.0 nM 7.70
CHEMBL4175878 Ki = 22.0 nM 7.66
CHEMBL4172715 Ki = 34.0 nM 7.47
CHEMBL4165484 Ki = 144.0 nM 6.84
CHEMBL4167125 KiH = 217.0 fM -
CHEMBL4172399 KiL = 1.73 nM -
CHEMBL4172399 KiH = 39.0 fM -
CHEMBL4161758 KiL = 3.82 nM -
CHEMBL4161758 KiH = 10.7 fM -
CHEMBL4167125 KiL = 0.68 nM -
CHEMBL4172264 KiL = 18.0 nM -
CHEMBL4172264 KiH = 10.6 fM -