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Assay Detail

CHEMBL4157376

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli GroEL expressed in Escherichia coli DH5alpha/Escherichia coli GroES expressed in Escherichia coli BL21 (DE3) assessed as reduction in GroEL/GroES-mediated denatured rhodanese refolding by measuring rhodanese enzyme activity after 45 mins by spectrometric analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 7 — Homologous single protein target
Curated By Intermediate

Target

GroEL/GroES (CHEMBL4106139)
Type PROTEIN COMPLEX
Organism Escherichia coli

Publication

Sulfonamido-2-arylbenzoxazole GroEL/ES Inhibitors as Potent Antibacterials against Methicillin-Resistant Staphylococcus aureus (MRSA).
Abdeen S, Kunkle T, Salim N, Ray AM, Mammadova N, Summers C, Stevens M, Ambrose AJ, Park Y, Schultz PG, Horwich AL, Hoang QQ, Chapman E, Johnson SM.
J Med Chem (2018) 61 : 7345 -7357
PubMed 30060666 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.10 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4161583 1 6.00
CHEMBL4167333 1 5.70
CHEMBL4176281 1 5.50
CHEMBL4160098 1 5.35
CHEMBL4162794 1 5.25
CHEMBL4166077 1 5.19
CHEMBL4161487 1 5.14
CHEMBL4168415 1 4.96
CHEMBL4176697 1 4.96
CHEMBL4169499 1 4.96
CHEMBL4168023 1 4.72
CHEMBL3955935 1 4.66
CHEMBL3975683 1 4.54
CHEMBL3970551 1 4.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4161583 IC50 = 1000.0 nM 6.00
CHEMBL4167333 IC50 = 2000.0 nM 5.70
CHEMBL4176281 IC50 = 3200.0 nM 5.50
CHEMBL4160098 IC50 = 4500.0 nM 5.35
CHEMBL4162794 IC50 = 5600.0 nM 5.25
CHEMBL4166077 IC50 = 6400.0 nM 5.19
CHEMBL4161487 IC50 = 7300.0 nM 5.14
CHEMBL4168415 IC50 = 11000.0 nM 4.96
CHEMBL4176697 IC50 = 11000.0 nM 4.96
CHEMBL4169499 IC50 = 11000.0 nM 4.96
CHEMBL4168023 IC50 = 19000.0 nM 4.72
CHEMBL3955935 IC50 = 22000.0 nM 4.66
CHEMBL3975683 IC50 = 29000.0 nM 4.54
CHEMBL3970551 IC50 = 33000.0 nM 4.48