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Assay Detail

CHEMBL4158337

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of c-terminal hexa-His tagged human S-COMT expressed in HEK293-6E cell membrane homogenate using 7,8-dihydroxy-4-methylcoumarin as substrate after 1 hr in presence of SAM by MTase glo methyltransferase assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Catechol O-methyltransferase (CHEMBL2023)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of 8-Hydroxyquinolines as Inhibitors of Catechol O-Methyltransferase.
Buchler I, Akuma D, Au V, Carr G, de León P, DePasquale M, Ernst G, Huang Y, Kimos M, Kolobova A, Poslusney M, Wei H, Swinnen D, Montel F, Moureau F, Jigorel E, Schulze MED, Wood M, Barrow JC.
J Med Chem (2018) 61 : 9647 -9665
PubMed 30272964 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.75 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4177089 1 6.30
CHEMBL4174102 1 6.30
CHEMBL4169861 1 6.30
CHEMBL4166574 1 5.90
CHEMBL4162990 1 5.80
CHEMBL4171117 1 5.80
CHEMBL4172614 1 5.80
CHEMBL4174978 1 5.70
CHEMBL4160303 1 5.70
CHEMBL4167128 1 5.60
CHEMBL4174117 1 5.50
CHEMBL4163421 1 5.50
CHEMBL4176890 1 5.20
CHEMBL4176008 1 5.10
CHEMBL4165800 1 -
CHEMBL4173001 1 -
CHEMBL4176829 1 -
CHEMBL4164621 1 -
CHEMBL4174751 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4177089 IC50 = 501.19 nM 6.30
CHEMBL4174102 IC50 = 501.19 nM 6.30
CHEMBL4169861 IC50 = 501.19 nM 6.30
CHEMBL4166574 IC50 = 1258.93 nM 5.90
CHEMBL4162990 IC50 = 1584.89 nM 5.80
CHEMBL4171117 IC50 = 1584.89 nM 5.80
CHEMBL4172614 IC50 = 1584.89 nM 5.80
CHEMBL4174978 IC50 = 1995.26 nM 5.70
CHEMBL4160303 IC50 = 1995.26 nM 5.70
CHEMBL4167128 IC50 = 2511.89 nM 5.60
CHEMBL4174117 IC50 = 3162.28 nM 5.50
CHEMBL4163421 IC50 = 3162.28 nM 5.50
CHEMBL4176890 IC50 = 6309.57 nM 5.20
CHEMBL4176008 IC50 = 7943.28 nM 5.10
CHEMBL4165800 IC50 > 10000.0 nM -
CHEMBL4173001 IC50 > 10000.0 nM -
CHEMBL4176829 IC50 > 10000.0 nM -
CHEMBL4164621 IC50 > 10000.0 nM -
CHEMBL4174751 IC50 > 10000.0 nM -