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Assay Detail

CHEMBL4177782

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal -His6 tagged FAAH (32 to 579 residues) expressed in Escherichia coli BL21 preincubated for 60 mins followed by olamide substrate addition measured every 10 sec intervals for 30 mins by spectrophotometry
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fatty-acid amide hydrolase 1 (CHEMBL2243)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Therapeutic Potential of Fatty Acid Amide Hydrolase, Monoacylglycerol Lipase, and N-Acylethanolamine Acid Amidase Inhibitors.
Tuo W, Leleu-Chavain N, Spencer J, Sansook S, Millet R, Chavatte P.
J Med Chem (2017) 60 : 4 -46
PubMed 27766867 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.41 7.79

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL560590 1 7.79
CHEMBL513789 1 7.48
CHEMBL184238 URB-597 1.0 1 6.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL560590 IC50 = 16.2 nM 7.79
CHEMBL513789 IC50 = 33.0 nM 7.48
CHEMBL184238 URB-597 IC50 = 109.0 nM 6.96