Assay Detail
Binding
CHEMBL4180216
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Displacement of Cy5-labelled (position-4) ATP from human HSP70 expressed in Escherichia coli BL21(DE3) after 16 hrs by microarray assay
6
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Development of a microarray-based assay for efficient testing of new HSP70/DnaK inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 6.04 | 9.00 |
| Kd | 2 | 6.54 | 7.43 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL14249 | ADENOSINE TRIPHOSPHATE | 2.0 | 2 | 9.00 |
| CHEMBL4217866 | — | — | 1 | 6.32 |
| CHEMBL4213277 | — | — | 1 | 5.64 |
| CHEMBL4206310 | — | — | 1 | 4.54 |
| CHEMBL4206826 | — | — | 1 | 4.31 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL14249 | ADENOSINE TRIPHOSPHATE | EC50 | = | 1.0 | nM | 9.00 |
| CHEMBL14249 | ADENOSINE TRIPHOSPHATE | Kd | = | 37.0 | nM | 7.43 |
| CHEMBL4217866 | — | EC50 | = | 484.0 | nM | 6.32 |
| CHEMBL4213277 | — | Kd | = | 2300.0 | nM | 5.64 |
| CHEMBL4206310 | — | EC50 | = | 29000.0 | nM | 4.54 |
| CHEMBL4206826 | — | EC50 | = | 49000.0 | nM | 4.31 |