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Assay Detail

CHEMBL4180809

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST-His6 tagged PDGFRalpha (Q551 to L1089 residues) expressed in Sf9 insect cells using poly(Ala,Glu,Lys,Tyr)6:2:5:1 hydrobromide as substrate in presence of [gamma-33P]ATP
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Platelet-derived growth factor receptor alpha (CHEMBL2007)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Trisubstituted purine inhibitors of PDGFRα and their antileukemic activity in the human eosinophilic cell line EOL-1.
Malínková V, Řezníčková E, Jorda R, Gucký T, Kryštof V.
Bioorg Med Chem (2017) 25 : 6523 -6535
PubMed 29089259 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 6.39 7.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4218356 1 7.23
CHEMBL4209971 1 7.22
CHEMBL4210919 1 7.18
CHEMBL4205604 1 7.18
CHEMBL4210474 1 7.02
CHEMBL4214422 1 6.82
CHEMBL4207053 1 6.81
CHEMBL4214362 1 6.81
CHEMBL4202703 1 6.80
CHEMBL4207048 1 6.73
CHEMBL4218779 1 6.71
CHEMBL4211413 1 6.54
CHEMBL4206527 1 6.34
CHEMBL4216607 1 5.96
CHEMBL4210897 1 5.91
CHEMBL4209694 1 5.85
CHEMBL4215315 1 5.83
CHEMBL4207999 1 5.50
CHEMBL4203120 1 5.32
CHEMBL4218942 1 5.29
CHEMBL4214781 1 5.12
CHEMBL367625 1 -
CHEMBL4205769 1 -
CHEMBL4218867 1 -
CHEMBL554 LAPATINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4218356 IC50 = 59.0 nM 7.23
CHEMBL4209971 IC50 = 60.0 nM 7.22
CHEMBL4210919 IC50 = 66.0 nM 7.18
CHEMBL4205604 IC50 = 66.0 nM 7.18
CHEMBL4210474 IC50 = 96.0 nM 7.02
CHEMBL4214422 IC50 = 150.0 nM 6.82
CHEMBL4207053 IC50 = 154.0 nM 6.81
CHEMBL4214362 IC50 = 156.0 nM 6.81
CHEMBL4202703 IC50 = 160.0 nM 6.80
CHEMBL4207048 IC50 = 187.0 nM 6.73
CHEMBL4218779 IC50 = 195.0 nM 6.71
CHEMBL4211413 IC50 = 290.0 nM 6.54
CHEMBL4206527 IC50 = 452.0 nM 6.34
CHEMBL4216607 IC50 = 1090.0 nM 5.96
CHEMBL4210897 IC50 = 1233.0 nM 5.91
CHEMBL4209694 IC50 = 1412.0 nM 5.85
CHEMBL4215315 IC50 = 1496.0 nM 5.83
CHEMBL4207999 IC50 = 3197.0 nM 5.50
CHEMBL4203120 IC50 = 4739.0 nM 5.32
CHEMBL4218942 IC50 = 5099.0 nM 5.29
CHEMBL4214781 IC50 = 7655.0 nM 5.12
CHEMBL367625 IC50 > 20000.0 nM -
CHEMBL4205769 IC50 > 20000.0 nM -
CHEMBL4218867 IC50 > 20000.0 nM -
CHEMBL554 LAPATINIB IC50 > 20000.0 nM -