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Assay Detail

CHEMBL4181957

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human CYP1B1 expressed in HEK293 cells using 7-ethoxyresorufin as substrate pretreated for 30 mins followed by substrate addition measured for 60 mins by fluorescence assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 1B1 (CHEMBL4878)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

(E)-3-(3,4,5-Trimethoxyphenyl)-1-(pyridin-4-yl)prop-2-en-1-one, a heterocyclic chalcone is a potent and selective CYP1A1 inhibitor and cancer chemopreventive agent.
Horley NJ, Beresford KJM, Kaduskar S, Joshi P, McCann GJP, Ruparelia KC, Williams IS, Gatchie L, Sonawane VR, Bharate SB, Chaudhuri B.
Bioorg Med Chem Lett (2017) 27 : 5409 -5414
PubMed 29138024 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.22 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2237710 1 7.66
CHEMBL4214111 1 7.51
CHEMBL4209004 1 7.22
CHEMBL4212722 1 7.22
CHEMBL283196 ALPHA-NAPHTHOFLAVONE 1 7.10
CHEMBL4202990 1 6.64
CHEMBL4213815 1 6.21
CHEMBL1568243 1 6.12
CHEMBL4210137 1 5.51
CHEMBL4210776 1 5.45
CHEMBL4205073 1 5.32
CHEMBL4218749 1 5.09
CHEMBL2237704 1 5.07
CHEMBL4211876 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2237710 IC50 = 22.0 nM 7.66
CHEMBL4214111 IC50 = 31.0 nM 7.51
CHEMBL4209004 IC50 = 60.0 nM 7.22
CHEMBL4212722 IC50 = 60.0 nM 7.22
CHEMBL283196 ALPHA-NAPHTHOFLAVONE IC50 = 80.0 nM 7.10
CHEMBL4202990 IC50 = 230.0 nM 6.64
CHEMBL4213815 IC50 = 610.0 nM 6.21
CHEMBL1568243 IC50 = 750.0 nM 6.12
CHEMBL4210137 IC50 = 3070.0 nM 5.51
CHEMBL4210776 IC50 = 3520.0 nM 5.45
CHEMBL4205073 IC50 = 4760.0 nM 5.32
CHEMBL4218749 IC50 = 8180.0 nM 5.09
CHEMBL2237704 IC50 = 8520.0 nM 5.07
CHEMBL4211876 IC50 = 9920.0 nM 5.00