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Assay Detail

CHEMBL4181977

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human CYP1A1 expressed in HEK293 cells assessed as reduction in benzo(a)pyrene-induced toxicity by measuring B[a]P EC50 at 2 times IC50 by MTT assay (Rvb = 0.8 +/- 0.14 uM)
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 1A1 (CHEMBL2231)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

(E)-3-(3,4,5-Trimethoxyphenyl)-1-(pyridin-4-yl)prop-2-en-1-one, a heterocyclic chalcone is a potent and selective CYP1A1 inhibitor and cancer chemopreventive agent.
Horley NJ, Beresford KJM, Kaduskar S, Joshi P, McCann GJP, Ruparelia KC, Williams IS, Gatchie L, Sonawane VR, Bharate SB, Chaudhuri B.
Bioorg Med Chem Lett (2017) 27 : 5409 -5414
PubMed 29138024 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 4.85 4.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4205073 1 4.85
CHEMBL4218749 1 4.85
CHEMBL4210137 1 4.85
CHEMBL4211876 1 4.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4205073 EC50 = 14000.0 nM 4.85
CHEMBL4218749 EC50 = 14000.0 nM 4.85
CHEMBL4210137 EC50 = 14000.0 nM 4.85
CHEMBL4211876 EC50 = 14000.0 nM 4.85