Assay Detail
Binding
CHEMBL4182153
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Inhibition of recombinant human N-terminal His6/SUMO-tagged PKM2 expressed in Escherichia coli BL21 using PEP as substrate incubated for 30 mins in presence of ADP by spectrophotometric based LDH enzyme coupled assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Publication
Scutellarin inhibits Hela cell growth and glycolysis by inhibiting the activity of pyruvate kinase M2.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 5.37 | 6.30 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL9470 | SHIKONIN | — | 1 | 6.30 |
| CHEMBL214344 | — | — | 1 | 4.90 |
| CHEMBL55415 | SCUTELLAREIN | — | 1 | 4.90 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL9470 | SHIKONIN | IC50 | = | 500.0 | nM | 6.30 |
| CHEMBL214344 | — | IC50 | = | 12500.0 | nM | 4.90 |
| CHEMBL55415 | SCUTELLAREIN | IC50 | = | 12500.0 | nM | 4.90 |