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Assay Detail

CHEMBL4183751

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of full length recombinant human PDE4B1 expressed in Sf21 insect cells using cAMP as substrate by scintillation proximity assay
26
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

3',5'-cyclic-AMP phosphodiesterase 4B (CHEMBL275)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Targeting a Subpocket in Trypanosoma brucei Phosphodiesterase B1 (TbrPDEB1) Enables the Structure-Based Discovery of Selective Inhibitors with Trypanocidal Activity.
Blaazer AR, Singh AK, de Heuvel E, Edink E, Orrling KM, Veerman JJN, van den Bergh T, Jansen C, Balasubramaniam E, Mooij WJ, Custers H, Sijm M, Tagoe DNA, Kalejaiye TD, Munday JC, Tenor H, Matheeussen A, Wijtmans M, Siderius M, de Graaf C, Maes L, de Koning HP, Bailey DS, Sterk GJ, de Esch IJP, Brown DG, Leurs R.
J Med Chem (2018) 61 : 3870 -3888
PubMed 29672041 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 26 5.91 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL193240 ROFLUMILAST 4.0 1 9.40
CHEMBL2326941 1 9.20
CHEMBL4202704 1 7.50
CHEMBL4202975 1 6.80
CHEMBL4210475 2 6.20
CHEMBL4218119 1 6.20
CHEMBL4209331 1 6.00
CHEMBL4214364 1 5.70
CHEMBL4204999 1 5.70
CHEMBL4218658 1 5.70
CHEMBL4206114 1 5.60
CHEMBL4217833 1 5.60
CHEMBL4206395 1 5.60
CHEMBL4204966 1 5.50
CHEMBL4218358 1 5.50
CHEMBL4213729 1 5.50
CHEMBL4208363 1 5.50
CHEMBL4214240 1 5.40
CHEMBL4209841 1 5.40
CHEMBL4213432 1 5.20
CHEMBL4213296 1 5.20
CHEMBL4205916 1 5.10
CHEMBL4216582 1 5.00
CHEMBL4203531 1 4.90
CHEMBL4209972 1 4.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL193240 ROFLUMILAST Ki = 0.3981 nM 9.40
CHEMBL2326941 Ki = 0.631 nM 9.20
CHEMBL4202704 Ki = 31.62 nM 7.50
CHEMBL4202975 Ki = 158.49 nM 6.80
CHEMBL4218119 Ki = 630.96 nM 6.20
CHEMBL4210475 Ki = 630.96 nM 6.20
CHEMBL4210475 Ki = 1000.0 nM 6.00
CHEMBL4209331 Ki = 1000.0 nM 6.00
CHEMBL4218658 Ki = 1995.26 nM 5.70
CHEMBL4214364 Ki = 1995.26 nM 5.70
CHEMBL4204999 Ki = 1995.26 nM 5.70
CHEMBL4217833 Ki = 2511.89 nM 5.60
CHEMBL4206114 Ki = 2511.89 nM 5.60
CHEMBL4206395 Ki = 2511.89 nM 5.60
CHEMBL4218358 Ki = 3162.28 nM 5.50
CHEMBL4204966 Ki = 3162.28 nM 5.50
CHEMBL4213729 Ki = 3162.28 nM 5.50
CHEMBL4208363 Ki = 3162.28 nM 5.50
CHEMBL4214240 Ki = 3981.07 nM 5.40
CHEMBL4209841 Ki = 3981.07 nM 5.40
CHEMBL4213432 Ki = 6309.57 nM 5.20
CHEMBL4213296 Ki = 6309.57 nM 5.20
CHEMBL4205916 Ki = 7943.28 nM 5.10
CHEMBL4216582 Ki = 10000.0 nM 5.00
CHEMBL4203531 Ki = 12589.25 nM 4.90
CHEMBL4209972 Ki = 50118.72 nM 4.30