Assay Detail
Binding
CHEMBL4184626
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [32P]S1P from recombinant human S1PR3 expressed in Chem1 cell membranes co-expressing Galpha15 pretreated for 30 mins followed by [32P]S1P addition measured after 60 mins by scintillation counting method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Design and synthesis of pyrazolopyridine derivatives as sphingosine 1-phosphate receptor 2 ligands.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 9.40 | 9.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL225155 | — | — | 1 | 9.40 |
| CHEMBL1368758 | — | — | 1 | - |
| CHEMBL4204756 | — | — | 1 | - |
| CHEMBL4212397 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL225155 | — | IC50 | = | 0.4 | nM | 9.40 |
| CHEMBL1368758 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL4204756 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL4212397 | — | IC50 | > | 1000.0 | nM | - |