Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4185007

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 20s constitutive proteasome beta5 chymotrypsin-like activity in human erythrocytes using Suc-Leu-Leu-Val-Tyr-AMC as substrate after 10 mins by fluorescence assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteasome Macropain subunit MB1 (CHEMBL4662)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of 2-thioxoimidazolidin-4-one derivatives as novel noncovalent proteasome and immunoproteasome inhibitors.
Maccari R, Ettari R, Adornato I, Naß A, Wolber G, Bitto A, Mannino F, Aliquò F, Bruno G, Nicolò F, Previti S, Grasso S, Zappalà M, Ottanà R.
Bioorg Med Chem Lett (2018) 28 : 278 -283
PubMed 29292224 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 5.66 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL64925 MG-132 1 8.40
CHEMBL4204445 1 4.87
CHEMBL4215753 1 4.75
CHEMBL4208777 1 4.63

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL64925 MG-132 Ki = 4.0 nM 8.40
CHEMBL4204445 Ki = 13600.0 nM 4.87
CHEMBL4215753 Ki = 17900.0 nM 4.75
CHEMBL4208777 Ki = 23600.0 nM 4.63