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Assay Detail

CHEMBL4189552

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full-length N-terminal GST-fused human PKC-alpha expressed in baculovirus expression system using (5FAM) RFARKGSLRQKNV as substrate after 60 mins by caliper assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein kinase C alpha type (CHEMBL299)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Fragment-Based Drug Discovery of Potent Protein Kinase C Iota Inhibitors.
Kwiatkowski J, Liu B, Tee DHY, Chen G, Ahmad NHB, Wong YX, Poh ZY, Ang SH, Tan ESW, Ong EH, Nurul Dinie, Poulsen A, Pendharkar V, Sangthongpitag K, Lee MA, Sepramaniam S, Ho SY, Cherian J, Hill J, Keller TH, Hung AW.
J Med Chem (2018) 61 : 4386 -4396
PubMed 29688013 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 6.60 7.35

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4212424 1 7.35
CHEMBL4208685 1 7.19
CHEMBL4202753 1 7.15
CHEMBL4216507 1 6.96
CHEMBL4206065 1 6.85
CHEMBL4208028 1 6.60
CHEMBL4213984 1 6.55
CHEMBL4206023 1 6.47
CHEMBL4211722 1 6.46
CHEMBL4215391 1 6.42
CHEMBL4212934 1 6.37
CHEMBL4214431 1 5.85
CHEMBL4203536 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4212424 IC50 = 45.0 nM 7.35
CHEMBL4208685 IC50 = 64.0 nM 7.19
CHEMBL4202753 IC50 = 71.0 nM 7.15
CHEMBL4216507 IC50 = 110.0 nM 6.96
CHEMBL4206065 IC50 = 140.0 nM 6.85
CHEMBL4208028 IC50 = 250.0 nM 6.60
CHEMBL4213984 IC50 = 280.0 nM 6.55
CHEMBL4206023 IC50 = 340.0 nM 6.47
CHEMBL4211722 IC50 = 350.0 nM 6.46
CHEMBL4215391 IC50 = 380.0 nM 6.42
CHEMBL4212934 IC50 = 430.0 nM 6.37
CHEMBL4214431 IC50 = 1400.0 nM 5.85
CHEMBL4203536 IC50 = 2500.0 nM 5.60